文献类型：期刊文献

英文题名：A Molecular Link in the Defence Response of Pinus massoniana to Pine Wilt Disease: Interactions Between TIFY Family Genes and the Nematode Effector

作者：Li, Yanshuo[1,2] Gao, Kai[1] Liu, Bin[1] Feng, Yi[1] Nie, Ziyan[1] Zhao, Yunxiao[1] Liu, Qinghua[1] Wang, Minyan[1] Li, Wei[2] Wang, Yangdong[1] Yin, Hengfu[1,2]

第一作者：Li, Yanshuo

通信作者：Wang, YD[1];Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Zhejiang Key Lab Forest Genet & Breeding, Hangzhou, Zhejiang, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Heilongjiang, Peoples R China

年份：2026

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-105029566195);WOS:【SCI-EXPANDED(收录号:WOS:001683656700001)】；

基金：Biological Breeding-National Science and Technology Major Project, Grant/Award Number: 2023ZD04059

语种：英文

外文关键词：BxCDP1 effector; Jasmonate pathway; JAZ/TIFY gene family; Pine Wilt Disease; Pinus massoniana

摘要：In response to pine wood nematode (PWN) invasion, pine trees can activate immune responses involving effector-triggered signalling, leading to redox imbalance and programmed cell death. However, such hypersensitive responses are also implicated in disrupting water conduction and accelerating wilting. Despite the identification of several PWN effectors, their roles in modulating host immunity remain unclear. Using integrated transcriptomic and metabolomic analyses across PWN infection timepoints, we revealed that the jasmonate (JA) pathway played a central role in the induction of defence responses; the expression levels of genes involved in JA biosynthesis and signal transduction changed markedly at different stages of PWN invasion. Through yeast two-hybrid screening, we revealed that the PWN effector BxCDP1 interacts with PmTIFY8, a key transcriptional regulator of the Jasmonate ZIM-domain (JAZ) family in the JA signalling pathway. We further demonstrated that this interaction occurs in the nucleus, attenuates reactive oxygen species (ROS)-mediated cell death, and modulates the expression of JA-responsive genes. Our results indicate that the interaction of BxCDP1 with JAZ proteins can impede the JA-mediated immune responses, which is a key link of PWN pathogenicity and provides information for genetic improvement to enhance resistance in pine trees.