文献类型：期刊文献

英文题名：Baseline Survey of Root-Associated Microbes of Taxus chinensis (Pilger) Rehd

作者：Zhang, Qian[1] Liu, Hongwei[1] Sun, Guiling[2] Wilson, Iain W.[3] Wu, Jianqiang[2] Hoffman, Angela[4] Cheng, Junwen[5] Qiu, Deyou[1]

第一作者：张倩

通信作者：Qiu, DY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Peoples R China;[3]CSIRO, Agr Flagship, Canberra, ACT 2001, Australia;[4]Univ Portland, Dept Chem, Portland, OR 97203 USA;[5]Zhejiang Forestry Acad, Key Lab Biol & Chem Utilizat Forest Resources, Hangzhou 310023, Zhejiang, Peoples R China

年份：2015

卷号：10

期号：3

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000352134700212)】；

基金：This work was supported by a grant for National non-profit Research Institutions of Chinese Academy of Forestry (CAFYBB2012042), NSFC grant 31300105 and a grant for the cooperation between Zhejiang Forrestry Academy and Chinese Academy of Forestry (2013SY17). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Taxol (paclitaxel) a diterpenoid is one of the most effective anticancer drugs identified. Biosynthesis of taxol was considered restricted to the Taxus genera until Stierle et al. discovered that an endophytic fungus isolated from Taxus brevifolia could independently synthesize taxol. Little is known about the mechanism of taxol biosynthesis in microbes, but it has been speculated that its biosynthesis may differ from plants. The microbiome from the roots of Taxus chinensis have been extensively investigated with culture-dependent methods to identify taxol synthesizing microbes, but not using culture independent methods., Using bar-coded high-throughput sequencing in combination with a metagenomics approach, we surveyed the microbial diversity and gene composition of the root-associated microbiomefrom Taxus chinensis (Pilger) Rehd. High-throughput amplicon sequencing revealed 187 fungal OTUs which is higher than any previously reported fungal number identified with the culture-dependent method, suggesting that T. chinensis roots harbor novel and diverse fungi. Some operational taxonomic units (OTU) identified were identical to reported microbe strains possessing the ability to synthesis taxol and several genes previously associated with taxol biosynthesis were identified through metagenomics analysis.