文献类型：期刊文献

英文题名：Establishment of Efficient Somatic Embryo Maturation System of Pinus elliottii

作者：Xu, Lin[1,2] Deng, Zhaolei[1,2] Hu, Shan[1,2] Liu, Qian[1,2] Luan, Qifu[3] Yang, Chunxia[1,2]

第一作者：Xu, Lin

通信作者：Yang, CX[1];Yang, CX[2]

机构：[1]Jiangxi Acad Forestry, Nanchang 330013, Peoples R China;[2]Jiangxi Prov Key Lab Improved Variety Breeding & E, Nanchang 330013, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2025

卷号：14

期号：13

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-105010581499);WOS:【SCI-EXPANDED(收录号:WOS:001525941300001)】；

基金：This work was supported by Biological Breeding-Major Projects (Grant No. 2023ZD04058), the National Natural Science Foundation of China (Grant No. 32160389), and the Key Research and Development Program of Jiangxi Province, China (Grant No. 20212BBF63018).

语种：英文

外文关键词：Pinus elliottii; somatic embryogenesis; somatic embryo maturation; hormone ratio; activated carbon

摘要：Pinus elliottii, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in Pinus elliottii, large-scale production remains challenging. Our results demonstrate that the genotype of Pinus elliottii significantly influences the induction rate of embryogenic callus. During somatic embryo maturation, the liquid-solid induction method increased the number of mature embryos by 25.85 times. Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS. Additionally, the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos. In the somatic embryo maturation stage, 1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus, resulting in a total of 1452 embryos. During germination, the application of 4 g/L activated carbon achieved a germination rate of 63%, and the survival rate of somatic embryo-derived seedlings reached 85%. This study not only identifies the optimal conditions for somatic embryogenesis in Pinus elliottii but also establishes an efficient protocol for somatic embryo maturation induction, providing a crucial scientific foundation for the rapid propagation and seedling production of Pinus elliottii.