文献类型：期刊文献

英文题名：Enhanced stability of thylakoid membrane proteins and antioxidant competence contribute to drought stress resistance in the tasg1 wheat stay-green mutant

作者：Tian, Fengxia[1] Gong, Jiangfeng[1] Zhang, Jin[2] Zhang, Meng[1] Wang, Guokun[1] Li, Aixiu[1] Wang, Wei[1]

第一作者：Tian, Fengxia

通信作者：Wang, W[1]

机构：[1]Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Tai An 271018, Shandong, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2013

卷号：64

期号：6

起止页码：1509-1520

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000317429500007)】；

基金：This work was supported by National Natural Science Foundation of China (No. 30671259).

语种：英文

外文关键词：Antioxidant competence; drought stress; stability; stay-green mutant; thylakoid membrane proteins; wheat

摘要：A wheat stay-green mutant, tasg1, was previously generated via mutation breeding of HS2, a common wheat cultivar (Triticum aestivum L.). Compared with wild-type (WT) plants, tasg1 exhibited delayed senescence indicated by the slower degradation of chlorophyll. In this study, the stability of proteins in thylakoid membranes was evaluated in tasg1 under drought stress compared with WT plants in the field as well as in seedlings in the laboratory. Drought stress was imposed by controlling irrigation and sheltering the plants from rain in the field, and by polyethylene glycol (PEG)-6000 in the laboratory. The results indicated that tasg1 plants could maintain higher Hill activity, actual efficiency ((PSII)), maximal photochemical efficiency of PSII (F-v/F-m), and Ca-2-ATPase and Mg-2-ATPase activities than the WT plants under drought stress. Furthermore, the abundance of some polypeptides in thylakoid membranes of tasg1 was greater than that in the WT under drought stress. Expression levels of TaLhcb4 and TaLhcb6 were higher in tasg1 compared with the WT. Under drought stress, the accumulation of superoxide radical (O-2) and hydrogen peroxide (H2O2) was lower in tasg1 compared with the WT not only at the senescence stage but also at the seedling stages. These results suggest greater functional stability of thylakoid membrane proteins in tasg1 compared with the WT, and the higher antioxidant competence of tasg1 may play an important role in the enhanced drought tolerance of tasg1.