文献类型：期刊文献

英文题名：Biochemical and molecular changes associated with heteroxylan biosynthesis in Neolamarckia cadamba (Rubiaceae) during xylogenesis

作者：Zhao, Xianhai[1,2] Ouyang, Kunxi[1,2] Gan, Siming[3] Zeng, Wei[4] Song, Lili[5] Zhao, Shuai[1,2] Li, Juncheng[1,2] Doblin, Monika S.[4] Bacic, Antony[4,6] Chen, Xiao-Yang[1,2] Marchant, Alan[7] Deng, Xiaomei[1,2,8] Wu, Ai-Min[1,2,8]

第一作者：Zhao, Xianhai

通信作者：Deng, XM[1]

机构：[1]South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Guangdong, Peoples R China;[2]State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Beijing, Peoples R China;[4]Univ Melbourne, Sch Bot, ARC Ctr Excellence Plant Cell Walls, Parkville, Vic 3052, Australia;[5]Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Nurturing Stn, Hangzhou, Zhejiang, Peoples R China;[6]Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3052, Australia;[7]Univ Southampton, Ctr Biol Sci, Southampton, Hants, England;[8]South China Agr Univ, Coll Forest, Guangzhou 510642, Guangdong, Peoples R China

年份：2014

卷号：5

期号：November

起止页码：1-14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000346969100001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Number 31170165), the special funds project of Forestry Public Welfare Industry Research of China (Grant Number 201004020), the Ministry of Science and Technology of China (2013AA102705), the Guangdong Natural Science foundation (Grant Number S2011010001138) in China. Wei Zeng, Monika. S. Doblin, and Antony Bacic acknowledge the support of the ARC Centre of Excellence in Plant Cell Walls, Australia (Grant Number CE110001007). We thank Cherie Walsh., from ARC Centre of Excellence in Plant Cell Walls, Australia, for cell wall linkage analysis.

语种：英文

外文关键词：heteroxylan; Neolamarckia cadamba (Rubiaceae); RNA-seq; XylT activity; xylogenesis

摘要：Wood, derived from plant secondary growth, is a commercially important material. Both cellulose and lignin assembly have been well studied during wood formation (xylogenesis), but heteroxylan biosynthesis is less well defined. Elucidation of the heteroxylan biosynthetic pathway is crucial to understand the mechanism of wood formation. Here, we use Neolamarckia cadamba, a fast-growing tropical tree, as a sample to analyze heteroxylan formation at the biochemical and molecular levels during wood formation. Analysis of the non-cellulosic polysaccharides isolated from N. cadamba stems shows that heteroxylans dominate non-cellulosic polysaccharides and increase with xylogenesis. Microsomes isolated from stems of 1-year-old N. cadamba exhibited UDP-Xyl synthase and xylosyltransferase activities with the highest activity present in the middle and basal stem regions. To further understand the genetic basis of heteroxylan synthesis, RNA sequencing (RNA-seq) was used to generate transcriptomes of N. cadamba during xylogenesis. The RNA-seq results showed that genes related to heteroxylan synthesis had higher expression levels in the middle and basal part of the stem compared to the apical part. Our results describe the heteroxylan distribution and heteroxylan synthesis trait in N. cadamba and give a new example for understanding the mechanism of heteroxylan synthesis in tropical tree species in future.