文献类型：期刊文献

英文题名：Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch

作者：Yan, Xuetong[1,2] Wang, Kejia[1,2] Zheng, Keyuan[2] Zhang, Lifeng[3] Ye, Yang[4,5] Qi, Liwang[3] Zhu, Mulan[1,2]

第一作者：Yan, Xuetong

通信作者：Zhu, ML[1];Zhu, ML[2];Qi, LW[3]

机构：[1]CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet NKLPMG, Shanghai 200032, Peoples R China;[2]Shanghai Key Lab Plant Funct Genom & Resources, Shanghai Chenshan Bot Garden, Shanghai 201602, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China;[5]Chinese Acad Sci, Shanghai Inst Mat Med, Dept Nat Prod Chem, Shanghai 201203, Peoples R China

年份：2023

卷号：3

期号：3-4

外文期刊名：FORESTRY RESEARCH

收录：Scopus(收录号：2-s2.0-85168809372);WOS:【ESCI(收录号:WOS:001379641000006)】；

基金：This work was supported by the National Science and Technology Major Projec of China (2018ZX08020003-005-001)

语种：英文

外文关键词：Larix olgensis; Mature zygotic embryo; Needle; Organogenesis; Taxifolin production

摘要：The deciduous conifer larch has been widely distributed around the world, is a high-quality wood species and is also used to extract industrial raw materials and medicines. In this study, we developed an organogenesis protocol for Larix olgensis from both mature zygotic embryos and needles, and analyzed the content of taxifolin in different tissues. The highest callus induction (96.8%) from mature zygotic embryo was found in acid (NAA), while from needles the highest callus induction (92.03%) was found in the Murashige and Skoog (MS) medium augmented with 3 mgL-1 6-BA and 0.3 mgL-1 NAA. The best shoot regeneration capacity from zygotic embryo-derived calli (83.3%) was obtained in DCR medium augmented with 1.0 mgL-1 6-BA and 0.01 mgL-1 NAA, and needle-derived calli were 77.3%. The shoots achieved the highest elongation (75.6%) in the DCR medium supplemented with 0.5 mgL-1 6-BA, 0.05 mgL-1 NAA and 2 gL-1 activated charcoal (AC). The rooting rate was 62.8% in DCR medium augmented with 3 mgL-1 Indole-3-butyric acid (IBA) and 100 mgL-1 phloroglucinol (PG). The accumulation of the taxifolin in elongation shoots and lignified elongation shoots have greatly improved along with the development process, were 28.6 mu gg-1, and 53 mu gg-1 respectively. The content of the taxifolin in callus was 1.99-5.26 mu gg-1, adventitious shoots were 4.8 mu gg-1, and adventitious roots were 2.86 mu gg-1. We report an efficient organogenesis and taxifolin production protocol in larch for the first time.