文献类型：期刊文献

中文题名：辽宁凤城市红松中松材线虫的分离与鉴定

英文题名：Isolation and identification of pine wood nematode in Pinus koraiensis in Fengcheng,Liaoning Province

作者：潘龙[1] 理永霞[1,2] 刘振凯[1] 孟繁丽[1] 陈军[3] 张星耀[1,2]

第一作者：潘龙

机构：[1]中国林业科学研究院林业新技术研究所,北京100091;[2]南京林业大学南方现代林业协同创新中心,江苏南京210037;[3]辽宁省凤城市林业有害生物防治检疫局,辽宁凤城118100

年份：2019

卷号：38

期号：1

起止页码：1-4

中文期刊名：中国森林病虫

外文期刊名：Forest Pest and Disease

收录：CSTPCD

基金："十三五"国家重点研发计划"林业有害生物检测;监测与预警关键技术"(2018YFD0600201)

语种：中文

中文关键词：松材线虫;红松;扩散型3龄幼虫

外文关键词：Bursaphelenchus xylophilus;Pinus koraiensis;third-stage dispersal juvenile

分类号：S763.16

摘要：近年来松材线虫Bursaphelenchus xylophilus在我国由南向北逐渐扩张并危害新的寄主。对辽宁省凤城市样地中红松Pinus koraiensis受害情况调查,利用显微镜形态观察和分子测序的方法对病原线虫进行综合鉴定。结果发现:凤城市样地中半枯和枯死红松体内均存在松材线虫,且87.7%以上为扩散型3龄幼虫,其分子鉴定结果与NCBI数据库中松材线虫的ITS和28S多个序列均完全相同,综合判断枯死红松中的病原线虫为松材线虫,由此确定松材线虫在我国自然条件下侵染红松。
In recent years,pine wood nematode has gradually expanded from south to north in China and harmed new hosts.The occurrence of pine wilt disease in the samples of Pinus koraiensis in Fengcheng,Liaoning Province was detected.Nematodes isolated from dead P.koraiensis were also identified by microscopic observation and molecular sequencing.The results showed that pine wood nematode was found in the P.koraiensis with yellowish needles,and more than 87.7%pine wood nematodes were third-stage dispersal juveniles.The result of molecular identification indicated that the ITS and 28S sequences of pine wood nematode identified were same as the sequences in the NCBI database.Through comprehensive judgment,the pathogenic nematode of dead P.koraiensis was pine wood nematode.The pine wood nematode began to invade P.koraiensis under natural conditions in China.