文献类型：期刊文献

英文题名：METHANE OXIDATION BACTERIA COMMUNITY STRUCTURES IN NANLING MOUNTAIN FOREST SOIL OF CHINESE CLIMATIC ECOTONE

作者：Zeng, Lile[1,2,3,4] Chen, Huai[1,2] NingWu[1,2,5] Yan, Zhiying[6] Du, Linfang[3] Shen, Yan[7] Wang, Xu[8] Tian, Jianqing[9] Luo, Yunpeng[10] Li, Wei[1,2,4] He, Yixin[1,2] Zhu, Dan[1,2]

第一作者：Zeng, Lile

通信作者：Chen, H[1]

机构：[1]Chinese Acad Sci, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;[2]Chinese Acad Sci, Ecol Restorat Biodivers Conservat Key Lab, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;[3]Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China;[4]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[5]Int Ctr Integrated Mt Dev, GPO Box 3226, Kathmandu, Nepal;[6]Chinese Acad Sci, Key Lab Environm & Appl Microbiol, CIB, Chengdu 610041, Sichuan, Peoples R China;[7]Cent South Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China;[8]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[9]Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China;[10]Northwest Agr & Forestry Univ, Yangling 712100, Peoples R China

年份：2018

卷号：27

期号：2

起止页码：1022-1030

外文期刊名：FRESENIUS ENVIRONMENTAL BULLETIN

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000427344600045)】；

基金：This study was supported by the International S&T Cooperation Program of China (S2013GI0408). The authors give special thanks to Ms. Wan Xiong for her editing and valuable comments on the manuscript, to Mr Zhijun Qiu and Wenqiang Hu for their help in samples collection and to Mrs. Dan Xue and LiFan Xiao for some soil chemical characteristics test.

语种：英文

外文关键词：CH4 oxidation; Forests soil; Methanotrophic community; Methylocaldum; climatic transition

摘要：Microbial oxidation is regarded as the only biological sink for atmospheric methane (CH4). The operators of methane oxidation in forest soils are still poorly understood, especially for forests in China. This study investigated the soils from Nanling Mountain forest near the Tropic of Cancer. All together 10 OTUs (Operational Taxonomic Unit) were obtained, with the ratio of type I/type II methanotrophs higher in the forest soil of south plot (similar to 1.6) than that of the north plots (0-0.9). Methylocaldum, Methylobacter-like/Upland Soil Cluster a Rold 1 (USC alpha R1), Tropical Upland Soil Cluster1 (TUSC1) dominate methane oxidation bacteria (MOB) community. Among them USC alpha contributed a bigger part in the north plots (about 50%) comparing with south plot (about 7%). The pmoA genes of dominant Methylocaldum MOB and those closely related to sequences of Methylocystis and Methylosinus MOB were detected in the south plot did not in the north plots. USC alpha R1, USC alpha R3 and Methylobacter-like methanotrophs tended to prefer soil environment of higher acidity. On the contrary, Upland Soil Cluster gamma methanotrophs tended to prefer neutral comparatively easier. The Shannon diversity index of methanotrophic community was positively related to pH but negatively related to bulk density (P < 0.05 for both), and the Simpson diversity index showed the similar tendency. The results indicate that there is heterogeneity of methanotrophic community in the Nanling climatic transition region.