文献类型：期刊文献

英文题名：Genome assembly and SSR molecular marker development and utilization in Sapindus mukorossi

作者：Li, Yongxiang[1] Wang, Zhaoshan[2] Shao, Wenhao[1] Sun, Kun[1] Zhou, Haoyu[1] Zhang, Tingyao[1] Jiang, Jingmin[1]

通信作者：Shao, WH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture State Forestry Adm, Beijing 100091, Peoples R China

年份：2025

卷号：226

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20250717884431);Scopus(收录号：2-s2.0-85217821939);WOS:【SCI-EXPANDED(收录号:WOS:001428715600001)】；

基金：This work was supported by Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02070-3-3) , National Science and Technology Basic Resources Survey Program of China (2019FY100803_02) , and Chinese Forestry Industry Standard Project (2023-LY-030) .

语种：英文

外文关键词：Sapindus mukorossi Gaertn.; Genome assembly; SSR markers; Genetic diversity

摘要：Sapindus mukorossi Gaertn. has garnered substantial attention from researchers both domestically and internationally owing to its considerable ecological, economic, and social significance. This paper employed the PacBio Sequel II platform sequencing in conjunction with second-generation paired-end sequencing to carry out the genome assembly and annotation of S. mukorossi. Furthermore, Simple Sequence Repeats (SSR) markers were developed to assess the genetic diversity within its germplasm resources. The overarching goal of these efforts is to establish a robust scientific basis for genomics research and molecular breeding of S. mukorossi. The assembly and correction process yielded a high-quality genome sequence of S. mukorossi comprising 390.25 Mb, and with 97.64 % of complete BUSCO genes (2071). The de novo annotation revealed that approximately 45.99 % of the genome consists of repetitive sequences, totaling 179.48 Mb. Using the S. mukorossi genome sequence, 20 pairs of high-quality SSR primers were developed and screened, exhibiting Polymorphism information content (PIC) values ranging from 0.73 to 0.91. The S. mukorossi germplasm resources displayed a significant level of genetic diversity. While substantial genetic differentiation was observed among different geographical origins, the primary source of genetic variation was within each origin. The results presented in this paper offer valuable insights for the collection and conservation of S. mukorossi germplasm resources. Additionally, these results provide a crucial foundation for future genomics research and molecular breeding efforts.