文献类型：期刊文献

中文题名：欧洲黑杨圆叶突变株系生理生化及DNA甲基化分析

英文题名：Physiological Characteristics and DNA Methylation Analysis of a Round-leaf Mutant of Populus nigra

作者：高亚南[1] 吴晓娟[1] 张伟溪[1] 苏晓华[1] 张冰玉[1]

第一作者：高亚南

机构：[1]中国林业科学研究院林业研究所

年份：2017

卷号：15

期号：11

起止页码：4647-4653

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：中国林科院基本科研业务费专项(CAFYBB2017ZA001-3)资助

语种：中文

中文关键词：欧洲黑杨N46;DNA甲基化;5-azaC;突变体;生理特性

外文关键词：P. nigra ‘N46＇, DNA methylation, 5-azaC, Mutant, Physiological characteristics

分类号：S0

摘要：为了创造杨树甲基化变异新种质,本研究以国外引进的优良欧洲黑杨种质资源N46(P.nigra'N46')无菌苗叶片为材料,通过在分化培养基中添加5-azaC(800μmol/L)的诱变方法,在再生植株群体中获得了一个圆叶突变株系M800-18。该株系叶片数量明显增多、叶片长宽比显著增大,叶绿素a、叶绿素b含量降低、光合能力下降,同时该株系保护酶活性显著增强,但其生长速度与对照基本相当。甲基化敏感扩增多态性分析(MSAP)结果表明,变异株系叶片基因组DNA甲基化水平均比对照显著升高。皮尔逊相关性分析结果表明,该株系DNA甲基化水平与叶片数量呈显著正相关,全甲基化、总甲基化水平与光合参数的各项指标均呈显著负相关,说明圆叶突变株系表型的变化很可能是由于甲基化的升高引起的。研究结果为研究杨树叶片形状变异的表观遗传基础研究及生产应用奠定了基础。
In or der to creat e DNA methylation mutant, in vitro leaves of P. nigra ‘N46＇were cultured on differentiation medium supplemented with 800 μmol/L 5-azacytidine（5-azaC） to induce methylation mutants. A round-leaf mutant（M800-18） was selected from the regenerated plantlets. Compared to wild type, the M800-18 lines had more leaves and higher length-to-width leaf ratio, whereas the content of both chlorophyll a and chlorophyll b decreased, which lead to the decline of photosynthetic capability. Meanwhile, the activities of peroxidase（POD） significantly enhanced. On the other hand, the growth speed of the two strains were similar. Analysis of methylation sensitive amplification polymorphism（MSAP） showed that the DNA methylation level of the mutant lines increased significantly compared to the control. Pearson correlation analysis indicated that the DNA methylation level of the mutant lines were positive correlated with the total leaf number, the levels of full-methylation and total methylation were significantly negatively correlated with various indicators of photosynthetic parameters. These results suggested that the phenotypic mutation of round-leaf lines were possibly caused by the increased level of DNA methylation. The round-leaf mutant obtained in this study provided valuable material for epigenetic study of leaf shape mutant, and created new germplasm resource for genetic breeding in poplar.