文献类型：期刊文献

英文题名：Screening and verification of EmMYB5 in flavonoids biosynthesis in Elaeagnus mollis dies kernel

作者：Feng, Yuqi[1] Song, Jia[2] Wang, Dongsheng[3] Hu, Xiaoyan[4] Li, Haoyang[1] Wang, Shengji[1] Zhao, Kai[1] Wu, Jing[5] Wang, Zhaoshan[6] Du, Shuhui[1]

第一作者：Feng, Yuqi

通信作者：Du, SH[1]

机构：[1]Shanxi Agr Univ, Coll Forestry, Jinzhong 030800, Shanxi, Peoples R China;[2]Powerchina Northwest Engn Corp Ltd, Xian 710000, Shaanxi, Peoples R China;[3]Hebei Normal Univ Sci & Technol, Engn Res Ctr Chestnut Ind Technol, Minist Educ, Qinhuangdao 066004, Peoples R China;[4]Shanxi Agr Univ, Coll Food Sci & Engn, Jinzhong 030800, Shanxi, Peoples R China;[5]Shanxi Acad Forestry & Grassland, Taiyuan 030000, Shanxi, Peoples R China;[6]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2025

卷号：236

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20254219330720);Scopus(收录号：2-s2.0-105018580969);WOS:【SCI-EXPANDED(收录号:WOS:001600151300002)】；

基金：This work was supported by Fundamental Research Project in Shanxi Province (202403021212071) , Special Project for Science and Tech-nology Cooperation and Exchange of Shanxi (202404041101034) and Open Fund of State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry (TGB-KF-2024-011) .

语种：英文

外文关键词：Elaeagnus mollis; Flavonoids biosynthesis; MYB transcription factor; Integrated analysis

摘要：Flavonoids are natural bioactive compounds with demonstrated health benefits in humans. Elaeagnus mollis Diels is a novel developing woody oil crop, and the flavonoids in E. mollis have attracted more attention. However, the composition content and biosynthesis mechanism of flavonoids in E. mollis kernel (EMF), the major application part, are still unclear. In this research, quantitative and multi-omics methods were orchestrated to explore dynamic and biosynthesis mechanism of flavonoids in EMF. The flavonoids content in EMF at nine harvest times (EMF1-EMF9) varied between 8.062 and 24.736 mg/g, peaking at EMF2. Multi-omics integration identified 59 core flavonoids biosynthesis genes, 711 DAMs, and 7312 DEGs. Enrichment analysis highlighted flavonoid, flavone and flavanol biosynthesis pathways. WGCNA analysis illustrated that the MEblue showed remarkable association with flavonoids quantity, and DEGs in this module significantly enriched in biosynthesis of secondary metabolites pathway. Correlation networks identified EmMYB5 and EmMYB105 as key regulators. Collectively, the functional characterization through transient overexpression and VIGS assays demonstrated that EmMYB5 acted as a negative regulator of flavonoids biosynthesis in EMF, with yeast-one-hybrid confirming EmMYB5 binding to the Em4CL promoter. The implementation of this study dissects the underlying mechanism of flavonoids biosynthesis in EMF, and also lays the foundation for further biofortified functional foods development of E. mollis.