文献类型：期刊文献

英文题名：Tamarix hispida aquaporin ThPIP2;5 confers salt and osmotic stress tolerance to transgenic Tamarix and Arabidopsis

作者：Wang, Liuqiang[1,2] Zhang, Chunrui[1] Wang, Yanmin[3] Wang, Yucheng[1] Yang, Chuanping[1] Lu, Mengzhu[2] Wang, Chao[1]

第一作者：Wang, Liuqiang;王留强

通信作者：Wang, C[1];Lu, MZ[2]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat,State Key Lab Tr, Beijing 100091, Peoples R China;[3]Forestry Sci Res Inst Hellongjiang Prov, Key Lab Fast Growing Tree Cultivating Heilongjian, Harbin 150040, Heilongjiang, Peoples R China

年份：2018

卷号：152

起止页码：158-166

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000438659900016)】；

基金：This work was supported by the Fundamental Research Funds for the Central Universities (2572014CA12) and National Natural Science Foundation of China (No. 31300571 and 31600539). We also thank the editor and reviewers for their critical comments and thoughtful suggestions.

语种：英文

外文关键词：Abiotic stress; Aquaporin; ROS-scavenging capability; Tamarix hispida

摘要：Aquaporin (AQP) proteins constitute a large family of protein channels that facilitate the transport of water and small neutral solutes through biological membranes, which is important for plants to combat abiotic stress. However, the precise functions of AQP genes involved in abiotic stress are not completely understood in plants. In this study, ThPIP2;5, an AQP gene of the PIP2 subgroup, was cloned and characterized from Tamarix hispida. Cellular localization assays showed that ThPIP2;5 was localized to the plasma membrane. Transgenic Arabidopsis plants overexpressing ThPIP2;5 displayed improved seed germination rates and increased root growth and fresh weight gain under salt and osmotic stresses, indicating that ThPIP2;5 can improve abiotic stress tolerance. ThPIP2;5-overexpressing and RNAi-silencing T. hispida plants were generated using the transient transformation method and selected for gain- and loss-of ThPIP2;5 analyses, respectively. Overexpression of ThPIP2;5 in transgenic Tamarix and Arabidopsis plants increased ROS-scavenging capability, activities of antioxidant enzymes and proline contents; decreased malondialdehyde (MDA) and H2O2 contents; and reduced electrolyte leakage rates compared to equivalent controls under salt and osmotic stresses. Together, these data suggested that ThPIP2;5 plays an important physiological role in abiotic stress tolerance in transgenic plants by reducing ROS accumulation and membrane damage and increasing the activities of antioxidants.