文献类型：期刊文献

英文题名：Factors affecting rooting of in vitro shoots of walnut cultivars

作者：Pei, Dong[] Yuan, Lichai[] Wang, Qingming[] Gu, Ruisheng[]

第一作者：裴东

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Key Lab Silviculture, State Forestry Adm, Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

年份：2007

卷号：82

期号：2

起止页码：223-226

外文期刊名：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000245609900011)】；

语种：英文

摘要：Factors affecting the rooting of in vitro shoots were investigated using precocious walnut (Juglans regia L.) cv. Xinzaofeng. In vitro sub-culture was the most important step to achieve rooting of the shoots. Rooting of shoots was markedly affected by the method of induction, 3-indole-butyric acid (IBA) concentrations, light conditions, as well as shoot quality. Rooting rates of >= 75.4% were obtained with an optimised rooting protocol in which vigorous shoots were sub-cultured 45-times at 20 d intervals. These shoots appeared tender, with small leaves. A transfer method of root induction was applied and shoots were first induced on a semi-solidified, diluted Walnut Specific Medium (1/4 DKW) with 25 mu M IBA in the dark for 12 d at 25 degrees +/- 3 degrees C, then cultured on semi-solidified 1/4 DKW medium plus vermiculite, without IBA, with a 16 h photoperiod (53 mu mol m(-2) s(-1)). This protocol was extended to five other walnut cultivars, and rooting rates of >= 60.5% were achieved in all cases.