文献类型：期刊文献

英文题名：Identification of Rice LncRNAs and Their Roles in the Rice Blast Resistance Network Using Transcriptome and Translatome

作者：Shan, Xiaoliang[1] Xia, Shengge[1,2] Peng, Long[3,4] Tang, Cheng[1] Tao, Shentong[1] Baig, Ayesha[5] Zhao, Hongwei[1]

第一作者：Shan, Xiaoliang

通信作者：Zhao, HW[1];Baig, A[2]

机构：[1]Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 210095, Peoples R China;[2]Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[5]COMSATS Univ Islamabad, Dept Biotechnol, Abbottabad Campus, Abbottabad 22060, Pakistan

年份：2025

卷号：14

期号：17

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-105015991165);WOS:【SCI-EXPANDED(收录号:WOS:001569746500001)】；

基金：This research was funded by the National Natural Science Foundation of China (grant number 32372556) and the Yangtze River Delta Science and Technology Innovation Consortium Key Research Project (grant number 24CSJ140200), awarded to H.Z.

语种：英文

外文关键词：long non-coding RNAs; Magnaporthe oryzae; plant immunity; ceRNA; WGCNA; hormone signaling

摘要：Long non-coding RNAs (lncRNAs) have emerged as pivotal regulators in plant immune responses, yet their roles in rice resistance against Magnaporthe oryzae (M. oryzae) remain inadequately explored. In this study, we integrated translatome data with conventional genome annotations to construct an optimized protein-coding dataset. Subsequently, we developed a robust pipeline ("RiceLncRNA") for the accurate identification of rice lncRNAs. Using strand-specific RNA-sequencing (ssRNA-seq) data from the resistant (IR25), susceptible (LTH), and Nipponbare (NPB) varieties under M. oryzae infection, we identified 9003 high-confidence lncRNAs, significantly improving identification accuracy over traditional methods. Among the differentially expressed lncRNAs (DELs), those unique to IR25 were enriched in the biosynthetic pathways of phenylalanine, tyrosine, and tryptophan, which suggests that they are associated with the production of salicylic acid (SA) and auxin (IAA) precursors, which may be involved in defense responses. Conversely, DELs specific to LTH primarily clustered within carbon metabolism pathways, indicating a metabolic reprogramming mechanism. Notably, 21 DELs responded concurrently in both IR25 and LTH at 12 h and 24 h post-inoculation, indicating a synergistic regulation of jasmonic acid (JA) and ethylene (ET) signaling while partially suppressing IAA pathways. Weighted gene co-expression network analysis (WGCNA) and competing endogenous RNA (ceRNA) network analysis revealed that key lncRNAs (e.g., LncRNA.9497.1) may function as miRNA "sponges", potentially influencing the expression of receptor-like kinases (RLKs), resistance (R) proteins, and hormone signaling pathways. The reliability of these findings was confirmed through qRT-PCR and cloning experiments. In summary, our study provides an optimized rice lncRNA annotation framework and reveals the mechanism by which lncRNAs enhance rice blast resistance through the regulation of hormone signaling pathways. These findings offer an important molecular basis for rice disease-resistant breeding.