文献类型：期刊文献

中文题名：^(13)CO_2示踪臭氧胁迫对水稻土壤微生物的影响

英文题名：Ozone Effects on Soil Microbial Community of Rice Investigated by ^(13)C Isotope Labeling

作者：陈展[1,2] 王效科[2] 尚鹤[1]

第一作者：陈展

机构：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室;[2]中国科学院生态环境研究中心城市与区域生态国家重点实验室

年份：2014

卷号：0

期号：10

起止页码：3911-3917

中文期刊名：环境科学

外文期刊名：Environmental Science

收录：MEDLINE(收录号：25693401);CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：国家自然科学基金项目(30670387)

语种：中文

中文关键词：臭氧;水稻;土壤微生物;13C同位素;13C-PLFA

外文关键词：ozone;;rice;;soil microbe;;13C isotope;;13C-PLFA

分类号：S511;X172

摘要：本研究采用具有日变化的熏蒸方式,利用13C同位素示踪方法,结合PLFA技术,模拟分析了臭氧浓度升高对水稻土壤微生物结构的影响.实验设置2个臭氧浓度处理,即空气对照(CF,臭氧浓度约4~10 nL·L-1),臭氧浓度升高处理(O3,8 h平均浓度为110 nL·L-1).分别在水稻熏气1个月和2个月时进行13C同位素标记实验.结果发现,臭氧熏蒸一个月的时候对土壤微生物生物量碳没有影响,但此时输入到土壤微生物的即时光合产物13C已经显著低于对照;随臭氧熏蒸时间的延长,无论是总的土壤微生物量碳还是即时光合产物13C对土壤微生物的输入都显著低于对照处理.PLFA主成分分析结果表明,熏蒸一个月时臭氧对13C-PLFA的结构组成并没有显著影响,但熏蒸两个月后则臭氧明显改变了13C-PLFA的组成,但两次标记后PLFA单体的Δδ13C‰值已受到臭氧的影响.臭氧胁迫前期,只有细菌脂肪酸的13C分配(13C%)受到臭氧的明显影响,而胁迫后期则臭氧主要影响的是分配给放线菌和真菌的13C.
This study was initiated to explore the effects of dynamic ozone( O3) exposure on soil microbial biomass and phospholipid fatty acids( PLFAs) under potted rice. A pulse-chase labeling experiment was designed to expose potted rice with13CO2 for 6 h after one and two months,the rice were fumigated by elevated O3 concentration with an 8 h mean of 110 nL·L- 1( O3). The allocation of the assimilated13 C to soil microorganisms was estimated by analyzing the13 C profile of microbial phospholipid fatty acids( PLFAs). After one month O3 exposure,the soil microbial biomass carbon was not affected,while the13C-microbial biomass was significantly decreased with elevated O3. Both the total and13 C microbial biomass carbon was remarkably lower than that of control treatment after two months O3 exposure. Principal components analysis of13C-PLFA data showed that elevated O3 significantly changed soil microbial structure after two month exposures,while there was no difference of13C-PLFA structure between control and elevated O3 treatments after one month exposure. Δδ13C ‰ of individual PLFA was significantly affected by O3 after both one and two month exposures. Only did ozone change the relative abundance of individual13C-PLFA(13C%) of bacterial fatty acids after one month exposure,while after two month exposures,the13C% of fungal and actinomycetic fatty acids were markedly changed by elevated O3.