文献类型：期刊文献

中文题名：北方地区黑杨派栽培品种的AFLP指纹分析

英文题名：AFLP Fingerprinting for 10 Cultivars of Section Aigeiros Widely Planted in North China

作者：李金花[1] 宋红竹[2] 周春江[3] 牛正田[1] 张绮纹[1] 王斌[3]

第一作者：李金花

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]国家林业局科技发展中心,北京100714;[3]中国科学院遗传与发育生物学研究所,北京100101

年份：2009

卷号：22

期号：2

起止页码：207-212

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家林业局标准制定项目“杨树新品种分子指纹图谱”([2001]林科标002号)

语种：中文

中文关键词：杨属;黑杨派;形态分类;AFLP;DNA指纹;品种鉴定

外文关键词：Populus ; section Aigeiros ; morphology; AFLP （ Amplified Fragment Length Polymorphism ） ; DNA fingerprinting; cultivar identification

分类号：S722.3

摘要：以我国北方地区杨树人工林广泛种植的10个黑杨派品种(36杨、50杨、107杨、108杨、111杨、加杨、沙兰杨、I-214杨、109杨和110杨)为实验材料,先进行了苗期形态特征分析,并利用AFLP标记技术,选用E-TA/M-CAG和E-TC/M-CAG两对选择性扩增引物组合,获得了12条特异性条带,建立了这10个杨树品种的DNA指纹图谱,每个品种的扩增带型互不相同,可为杨树品种或无性系鉴定提供独特的特征。结果表明:利用AFLP DNA指纹图谱可以灵敏地鉴别相近无性系并与形态学鉴别分类结论相吻合,表明DNA指纹鉴定配合形态学鉴定方法具有较高的准确性和有效性。
Ten cultivars of the section Aigeiros widely planted in North China for poplar culture and intensive forest plantations were selected for AFLP fingerprinting, including P. deltoides Bartr. cl ‘2KEN8＇ , P. deltoides Marsh. cl. ‘ Drava＇ （ 55/65 ）, P. x euramericana Moench cl. ‘ Neva＇ （ 74/76 ）, P. x euramericana Moench cl ‘ Guariento＇ , P. x euramericana Moench cl ‘ Bellotto＇ , P. x euramericana Moench cl.‘ Jiayang＇ , P. x euramericana Moench cl ‘ Sacrau-79＇ , P. x ‘Mincio＇, P. deltoides Marsh. x P. maximowiczii Henry cultivars were used for morphological analysis in nursery. Moench cl ‘I-214＇, P. deltoides x P. alba cl. cl. ‘Eridano＇. One-year-old plantlets of these ten As the results of AFLP fingerprinting for these ten cultivars, 12 polymorphic bands were amplified with two primer combinations E-TA/M-CAG and E-TC/M-CAG respectively. Based on the 12 reliable AFLP polymorphic bands, the DNA fingerprint was constructed to identify these 10 cuhivars. In this DNA fingerprint, each genotype had its unique finger pattern and could be easily distinguished from the others. DNA fingerprinting analysis provides a good method for cuhivars identification and can overcome the limitations associated with morphological and origin data.