文献类型：期刊文献

英文题名：Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses

作者：Zhang Y. Han X. Chen S. Zheng L. He X. Liu M. Qiao G. Wang Y. Zhuo R.

第一作者：Zhang Y.

机构：[1]State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China;[2]Key Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, 311400, China;[3]School of Architectural and Artistic Design, Henan Polytechnic University, Jiaozuo, Henan, 454000, China;[4]College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan, 650201, China

年份：2017

卷号：7

外文期刊名：Scientific Reports

收录：Scopus(收录号：2-s2.0-85010791679)

语种：英文

摘要：Salix matsudana is a deciduous, rapidly growing willow species commonly cultivated in China, which can tolerate drought, salt, and heavy metal stress conditions. Selection of suitable reference genes for quantitative real-time PCR is important for normalizing the expression of the key genes associated with various stresses. To validate suitable reference genes, we selected 11 candidate reference genes (five traditional housekeeping genes and six novel genes) and analyzed their expression stability in various samples, including different tissues and under different abiotic stress treatments. The expression of these genes was determined using five programs-geNorm, NormFinder, BestKeeper, ? "Ct, and RefFinder. The results showed that α-TUB2 (alpha-tubulin 2) and DnaJ (chaperone protein DnaJ 49) were the most stable reference genes across all the tested samples. We measured the expression profiles of the defense response gene SmCAT (catalase) using the two most stable and one least stable reference genes in all samples of S. matsudana. The relative quantification of SmCAT varied greatly according to the different reference genes. We propose that α-TUB2 and DnaJ should be the preferred reference genes for normalization and quantification of transcript levels in future gene expression studies in willow species under various abiotic stress conditions. ? 2017 The Author(s).