文献类型：期刊文献

英文题名：Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse

作者：Bian, Jing[1] Peng, Feng[1,2] Peng, Xiao-Peng[3,4] Peng, Pai[2] Xu, Feng[1] Sun, Run-Cang[1,2]

第一作者：Bian, Jing

通信作者：Sun, RC[1]

机构：[1]Beijing Forestry Univ, Inst Biomass Chem & Technol, Beijing 100083, Peoples R China;[2]S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China

年份：2013

卷号：127

起止页码：236-241

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20130215880889);Scopus(收录号：2-s2.0-84868244944);WOS:【SCI-EXPANDED(收录号:WOS:000312926400033)】；

基金：The authors are grateful to grants from the Fundamental Research Funds for the Central Universities (BLYJ201215), Natural Science Foundation of China (No. 30930073), and the Ministry of Science and Technology (973, 2010CB732204).

语种：英文

外文关键词：Sugarcane bagasse; Xylan; Xylooligosaccharides; Xylanase; Antioxidant activity

摘要：Xylooligosaccharides (XOS) were prepared from xylan-rich hemicelluloses isolated by potassium hydroxide from sugarcane bagasse by hydrolysis with crude xylanase secreted by Pichia stipitis. Hydrolysis for 12 h produced XOS with a maximum yield of 31.8%, equivalent to 5.29 mg mL(-1) in the hydrolyzate. XOS with degrees of polymerization (DP) from 2 to 4 (xylobiose, xylotriose, and xylotetraose) were the major components in the hydrolysates, whereas the oligosaccharides with higher DP of 5-6 (xylopentaose and xylohexose) showed a constant low level. FT-IR and NMR (H-1, C-13, HSQC) demonstrated that XOS contained Araf and 4-O-Me-alpha-D-GlcpA residues. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that the XOS exhibited concentration-dependent antioxidant activity. The results obtained indicate that the XOS produced from sugarcane bagasse can be employed in food-related applications. (C) 2012 Elsevier Ltd. All rights reserved.