文献类型：期刊文献

中文题名：筛胸梳爪叩甲(Melanotus cribricollis)幼虫共生细菌对绿僵菌的抑菌活性

英文题名：Antimicrobial activity of endosymbiotic bacteria isolated from Melanotus cribricollis larva against Metarhizium pingshaense

作者：方林鑫[1] 李志红[1] 张亚波[1] 张威[1] 舒金平[1] 王浩杰[1]

第一作者：方林鑫

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400

年份：2021

卷号：40

期号：12

起止页码：3990-3997

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2019SY015)资助。

语种：中文

中文关键词：筛胸梳爪叩甲;次生共生菌;挥发物;抑菌活性;生物防治

外文关键词：Melanotus cribricollis;secondary endosymbiont;volatile;antimicrobial activity;biological control

分类号：S763.7

摘要：绿僵菌是重要的害虫生防资源,其防治效果常受到环境中其他微生物的影响,而其内在机制尚不明晰。本研究测定了筛胸梳爪叩甲(Melanotus cribricollis)幼虫体内次生内共生菌——吉伯伦不动杆菌(Acinetobacter gyllenbergii)对平沙绿僵菌(Metarhizium pingshaense)活性的抑制作用。结果表明,不动杆菌发酵液与挥发物对平沙绿僵菌孢子萌发及产孢量均有极显著的抑制效果;发酵液对菌丝生长存在抑制作用,但作用时间较短,至处理后第7天,这种抑制效果即不明显,挥发物则对菌丝生长无影响;利用气相色谱质谱联用仪(GC-MS)分析筛选不动杆菌9种挥发性物质,其中α-蒎烯、苯胺、壬醛、2,4二叔丁基苯酚、柠檬烯、1-十二烯和二甲基三硫对绿僵菌活性具有显著的抑制作用。金针虫内共生菌吉伯伦不动杆菌对绿僵菌活性具有强烈的抑制作用。
Metarhiziumspp. is an important biocontrol resource for the management of underground pests. While it is known that the control efficiency as entomopathogenic fungi is often affected by other microorganisms, the underlying mechanism is unclear. In this study, we examined the inhibitory effect ofAcinetobacter gyllenbergii, a secondary endosymbiont isolated from Melanotus cribricollislarva, on the activity ofMetarhizium pingshaensethat effectively infects wireworm. The results showed that both the fermentation broth and volatiles ofA. gyllenbergii showed significant inhibitory effects on spore germination and production ofM. pingshaense.The fermentation broth ofA. gyllenbergiisignificantly inhibited mycelium growth ofM. pingshaensein seven days, while the volatiles ofA. gyllenbergiidid not affect mycelium growth ofM. pingshaense. Nine effective volatile compounds ofA. gyllenbergiiwere identified by GC-MS analysis.Seven compounds, inclduing α-pinene, aniline, nonanal, 2,4-di-tert-butylphenol, limonene, 1-dodecene and dimethyl trisulfide, had significant inhibitory effects on the activity ofM. pingshaense. Therefore, the endosymbiotic bacteria ofM. cribricollislarva showed a strong inhibitory effect on the growth ofM. pingshaense.