文献类型：期刊文献

英文题名：Towards an efficient regeneration protocol for eucalyptus urophylla

作者：Li, L.M.[1] Ouyang, L.J.[1] Gan, S.M.[2,3]

第一作者：Li, L.M.

机构：[1] Guangdong University of Petrochemical Technology, Maoming, 525000, China; [2] State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China; [3] Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China

年份：2015

卷号：27

期号：3

起止页码：289-297

外文期刊名：Journal of Tropical Forest Science

收录：EI(收录号：20160201801186);Scopus(收录号：2-s2.0-84953867348)

语种：英文

外文关键词：pH - Metabolism - Naphthalene - Polycyclic aromatic hydrocarbons - Acetic acid - Butyric acid - Reforestation

摘要：The aim of the present study was to establish an efficient regeneration system for Eucalyptus urophylla by means of organogenesis. The hypocotyls from seedlings of E. urophylla clone U6 were used as explants and cultured in a modified Murashige and Skoog (MS) medium, supplemented with 13.2 μM L-1 N-phenyl- N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285μM L-1 indole-3-acetic acid (IAA). After culture for 5 days, 98.8% explants formed callus. After 30 days, the calli obtained were transferred to Schwarz differential medium (SDM) containing different combinations of 6-benzyladenine (6-BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage (58.6%) of the calli induced by PBU showed adventitious bud formation. Proliferation of adventitious buds was obtained on SDM medium supplemented with 2.2 μM L-1 6-BA and 0.25 μM L-1 NAA and shoot elongation was then stimulated on SDM medium supplemented with 5.28 μM L-1PBU and 0.25 μM L-1 NAA for 20 days. For rooting, the elongated shoots were cultivated on root induction medium containing 2.46 μM L-1 indole-3-butyric acid (IBA). Plantlets were then successfully transplanted to a greenhouse. This paper presents an efficient procedure for regeneration of E. urophylla. ? Forest Research Institute Malaysia.