文献类型：期刊文献

英文题名：Isolation of Tulasnella spp. from Cultivated Paphiopedilum Orchids and Screening of Germination-Enhancing Fungi

作者：Yao, Na[1] Zheng, Baoqiang[1] Wang, Tao[2] Cao, Xiaolu[1]

第一作者：姚娜

通信作者：Cao, XL[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry & G, Beijing 100091, Peoples R China;[2]Beijing Floriculture Engn Technol Res Ctr, Beijing Lab Urban & Rural Ecol Environm, China Natl Bot Garden North Garden, Beijing 100093, Peoples R China

年份：2023

卷号：9

期号：6

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001015293400001)】；

语种：英文

外文关键词：orchid mycorrhizal fungi; symbiosis; Orchidaceae; conservation

摘要：Ex situ conservation, an important way to increase the survival and sustainability of endangered species, is widely used in the conservation of endangered orchids. However, long-term ex situ conservation might affect the dominant group of orchid symbiotic fungi, which are crucial for orchid growth and reintroduction. This study investigated the culturable Tulasnella spp. associated with Paphiopedilum orchids after long-term greenhouse cultivation, and identified germination-enhancing isolates. A total of 44 Tulasnella isolates were obtained from the roots of 14 Paphiopedilum spp., and 29 of them were selected for phylogenetic analysis. They clustered mainly with Tulasnella deliquescens, Tulasnella calospora, Tulasnella bifrons, and Tulasnella irregularis, but included two potential new groups. Compared with published uncultured data, most of the isolates were grouped together with the reported types, and the dominant Tulasnella associated with P. armeniacum and P. micranthum could still be isolated after ten years of cultivation, most of which were the first isolation. In vitro symbiotic germination showed that certain root isolates could promote seed germination (e.g., parm152 isolated from P. armeniacum, Php12 from P. hirsutissimum, and prhi68 from P. rhizomatosum). These data indicated that the dominant Tulasnella types colonizing the roots of cultivated Paphiopedilum are stable over time, and germination-enhancing fungi colonizing the roots would benefit for seed reproduction after population reintroduction into the wild.