文献类型：期刊文献

中文题名：碱性过氧化氢预处理对慈竹木质素物化性质及结构的影响

英文题名：Effect of Alkaline Hydrogen Peroxide Pretreatment on the Physicochemical Properties and Structure of Bambusa emeiensis Lignin

作者：詹云妮[1,2] 黄晨[1] 焦健[1] 周雪莲[1,2] 吕焱[1] 房桂干[1,2]

第一作者：詹云妮

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏南京210042;[2]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037

年份：2025

卷号：45

期号：6

起止页码：21-30

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：;北大核心:【北大核心2023】；

基金：浙江省省院合作林业科技项目(2024SY06);江苏省生物质能源与材料重点实验室基本科研业务费(JSBEM-S-202309);宜宾市竹产业重大攻关项目(YBZD202401)。

语种：中文

中文关键词：慈竹;碱性过氧化氢预处理;回收木质素;热稳定性;抗氧化活性

外文关键词：Bambusa emeiensis;alkaline hydrogen peroxide pretreatment;recovered lignin;thermal stability;antioxidant activity

分类号：TQ35

摘要：采用碱性过氧化氢(AHP)体系对慈竹进行预处理,并通过酸沉析法从预处理液中回收木质素,实现木质素于温和条件下的有效分离。利用傅里叶变换红外光谱(FT-IR)、凝胶渗透色谱(GPC)、二维核磁共振技术(2D-HSQC NMR)和热重(TG)分析仪对回收木质素(L_(0)~L_(3.0))结构进行表征,并对木质素的抗氧化活性进行评价。研究结果表明:AHP预处理可实现木质素的有效脱除,脱除率最高达70.97%(3.0%H_(2)O_(2)),得率最高达60.18%(0%H_(2)O_(2))。2D-HSQC谱图分析表明:回收木质素的结构保留较完整,主要由紫丁香基(S)单元和愈创木基(G)单元组成,S和G的比值为1.45~1.50;回收木质素中β-O-4'键(A)占比高达54.76%~59.84%(以苯环计,下同),β-β'(B)和β-5'(C)占比分别为3.04%~4.10%和1.32%~2.73%。GPC结果表明:与酶解木质素(CEL)相比,回收木质素的相对分子质量降低,多分散性指数(PDI)在4.80左右。此外,与CEL相比,随着H_(2)O_(2)质量分数由0%增至2.0%,预处理液中回收木质素均表现出良好的热稳定性和抗氧化活性,其热分解主要发生在250~400℃之间,对DPPH自由基的半数抑制质量浓度(IC_(50))值分别为17.62(L_(0))、16.26(L_(1.0))和15.99(L_(2.0))mg/L,低于CEL的28.97 mg/L。
Alkaline hydrogen peroxide(AHP)pretreatment was used to pretreat bamboo(Bambusa emeiensis),and the recovered lignin was obtained from the AHP pretreatment liquor by acid precipitation,realizing the mild and effective lignin separation.The structural properties of recovered lignin(L_(0)-L_(3.0))were investigated with Fourier transform infrared spectroscopy(FT-IR),gel permeation chromatography(GPC),two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance(2DHSQC NMR)and thermogravimetric analysis(TG).Moreover,the antioxidant activity of recovered lignin was also evaluated.The results showed that the AHP pretreatment could remove lignin effectively,the maximum of lignin removal yield was 70.97%at 3.0%H_(2)O_(2)dosage,and the yield of lignin samples was up to 60.18%at 0%H_(2)O_(2).The 2D-HSQC NMR spectra showed that the structure of removed lignin was well preserved,which mainly composed of syringyl(S)and guaiacyl units(G),and the values of S/G were 1.45-1.50.In addition,a predominance ofβ-O-4'linkages up to 54.76%-59.84%,the contents ofβ-β'(B)andβ-5'(C)linkages were 3.04%-4.10%and 1.32%-2.73%,respectively.Gel permeation chromatography showed that compared to cellulolytic enzyme lignin(CEL),the relative molecular mass of removed lignin was lower,and the polydispersity index(PDI)was stable at around 4.80.Furthermore,as the dosage of H_(2)O_(2)increased from 0%to 2.0%,the removed lignin exhibited favorable thermal stability and antioxidant activity.Most of them degraded in the temperature range of 250-400℃,and the half maximal inhibitory concentrations(IC_(50))values of DPPH free radical scavenging ability were 17.62(L_(0)),16.26(L_(1.0)),and 15.99(L_(2.0))mg/L,respectively,which were lower than 28.97 mg/L of CEL.