文献类型：期刊文献

英文题名：Proteins responding to drought and high-temperature stress in Pinus armandii Franch

作者：He, Cai-Yun[1] Zhang, Jian-Guo[1] Duan, Ai-Guo[1] Sun, Hong-Gang[1] Fu, Li-Hua[1] Zheng, Shu-Xing[1]

第一作者：何彩云

通信作者：Zhang, JG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Silviculture, Beijing 100091, Peoples R China

年份：2007

卷号：85

期号：10

起止页码：994-1002

外文期刊名：CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000252310000011)】；

语种：英文

外文关键词：Pinus armandii Franch; drought; high temperature; proteomics

摘要：Proteomic analysis provides a powerful method for studying plant responses to stress at the protein level. To study stress-responsive molecular mechanisms for Pinus armandii Franch, one of the most important forest plantation tree species in subalpine regions of Asia, we analyzed the response of 2-year-old P. armandii seedlings to drought and high temperature using two-dimensional gel electrophoresis. More than 550 reproducible needle proteins were detected in the controls and treatments, and the abundance of 27 proteins were found to change noticeably. We identified five proteins affected by drought stress and eight proteins affected by high temperature. These proteins are functionally quite diverse and are involved in photosynthesis, cell division and elongation, antioxidant metabolism, ammonia assimilation, growth and development, and protein folding. Our results provide fundamental data for future research on responses to drought and high temperature. As drought and high temperature are two major factors limiting the growth of subalpine forests during summer under recent global warming, this research may contribute to an understanding of the development of stress tolerance in trees.