文献类型：期刊文献

中文题名：百合查尔酮合成酶基因克隆及其转化烟草的花色表达分析

英文题名：Cloning of Chalcone Synthase Gene in Lilium and Expression Analysis of Flower Colour Changes in Transgenic Tobacco

作者：陈洁[1,2] 安利清[2] 王涛[3] 姚娜[1] 李潞滨[1] 杨凯[2]

第一作者：陈洁

机构：[1]中国林业科学研究院林业研究所林木遗传育种国家重点实验室;[2]北京农学院农业应用新技术北京市重点实验室;[3]北京林业大学园林学院

年份：2012

卷号：32

期号：8

起止页码：1511-1517

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金："十二五"国家科技支撑计划课题(2012BAD01B07)

语种：中文

中文关键词：查尔酮合成酶基因(CHS);载体构建;烟草转化;类黄酮;花色改变

外文关键词：, chalcone synthase gene （CHS）; vector construction;tobacco transformation; flavonoids; color-altered flowers

分类号：Q789

摘要：以‘西伯利亚’百合为试材,利用PCR技术克隆了查尔酮合成酶基因(CHS),构建了CHS基因的正义和反义植物表达载体,采用农杆菌介导法转化烟草叶盘,获得了转正义CHS基因的本明烟草18株,转反义CHS基因的普通烟草21株,总转化率为26.0%。高效液相色谱法(HPLC)检测结果显示,正义CHS转基因的本明烟草类黄酮含量升高14.0%～59.7%,反义CHS转基因的普通烟草类黄酮含量降低44.5%～76.4%。花色观察结果显示,正义转基因烟草的花瓣颜色未见变化,反义转基因烟草部分植株的花瓣颜色变浅。研究表明,CHS基因遗传转化是进行花色调控的有效手段之一。
The chalcone synthase gene （CHS） was cloned from Lilium oriental hybrid ＇Siberia＇ by PCR method. Sense and antisense CHS were constructed into plant expression vectors pCAMBIA1304,and then transformed into tobacco by agrobacterium mediated approach. 18 plants of sense CHS transgenic Nicotiana benthamiana and 21 plants of antisense CHS transgenic Nicotiana tabacum were obtained and the total transform ratio was 26.0%. High Performance IAquid Chromatography （HPLC） suggested that CHS expression accumulation amplified by 14.0%-59.7% in N. benthamiana transformed with sense gene, and the accumulation declined by 44.5%-76.4% in N. tabacum transformed with antisence gene eompared to the wild type. The petals of the transgenic N. benthamiana had no color changes, but the petal color in some of the transgenic N. tabacum plants was faded. The results indicated that the transformation of CHS genewas an effective method to flower color regulation.