文献类型：期刊文献

中文题名：联合固氮菌对桉树青枯病菌的抑制作用研究

英文题名：Inhibiting Effect of Associative Nitrogen-fixation Bacteria on Bacteria Wilt Disease of Eucalyptus

作者：马海宾[1] 康丽华[1] 江业根[1] 郑翠梅[1]

第一作者：马海宾

机构：[1]中国林业科学研究院热带林业研究所

年份：2007

卷号：20

期号：4

起止页码：473-476

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：林业科技支撑计划资助(2006BAD08A11);国家林业局中试星火项目;中国林科院基金项目(2004-15)

语种：中文

中文关键词：桉树;联合固氮菌;青枯病菌;抑菌作用

外文关键词：Eucalyptus ; associative nitrogen-fixation bacteria ; Ralstonia solanacearum ; inhibitory

分类号：S792.39

摘要：采用纸碟法和液体比浊法研究了联合固氮菌对桉树青枯病菌茄拉尔氏菌(Ralstonia solanacearum(E.F.Smith)Yabuuchi)的抑制作用。结果表明两株联合固氮菌1和固氮菌2及其培养物上清液对青枯菌Ra均有不同程度的抑制作用,上清液抑制效果好于菌悬液抑制效果,混合菌株的抑制作用高于单一菌株的抑制作用。温室接种结果表明,接种固氮菌1和同时接种固氮菌1及青枯菌Ra的处理比不接种对照(CK)桉树苗高增加33.19%和31.09%;单接种固氮菌1的桉树苗地上干质量比对照(CK)增加123.23%,同时接种固氮菌1及青枯菌Ra的桉树苗比对照(CK)地上干质量增加93.22%;接种固氮菌1和同时接种固氮菌1及青枯菌Ra的处理比对照(CK)桉树苗地下干质量分别增加101.52%和71.81%。接种固氮菌可以降低桉树苗木死亡率38.24%。桉树幼苗温室接种试验证明接种固氮菌的桉树苗木生长旺盛,茎干较对照粗壮,长势整齐,固氮菌可以提高桉树苗木对青枯病的抗性。
The inhibiting effect of associative nitrogen-fixation bacteria on bacteria wilt disease of Eucalyptus was examined. The growth of Ralstonia solanacearum was inhibited by both associative nitrogen-fixation bacteria（ No. 1 and No. 2） and culture filtrate of associative nitrogen-fixation bacteria （ No. 1 and No. 2 ）. Eucalyptus nursery experiments were established in the greenhouse to determine the controlling effect of associative nitrogen-fixation bacteria on bacteria wilt disease. The results showed that Eucalyptus seedlings inoculated with associative nitrogen-fixation bacteria and dual inoculated with associative nitrogen-fixation bacteria and Ralstonia solanacearum were higher than those without any inoculation. And the biomass above ground and underground of Eucalyptus seedlings inoculated with associative nitrogen-fixation bacteria were increased by 123.23% and 101.52% respectively while the biomass above ground and underground of Eucalyptus seedlings dual inoculated with associative nitrogen-fixation bacteria and Ralstonia solanacearum were increased by 93.22% and 71.81% respectively.