文献类型：期刊文献

英文题名：Proteomic analysis of stress-related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation

作者：Jing, Danlong[1] Zhang, Jianwei[1] Xia, Yan[1] Kong, Lisheng[2] OuYang, Fangqun[1] Zhang, Shougong[1] Zhang, Hanguo[3] Wang, Junhui[1]

第一作者：Jing, Danlong

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing, Peoples R China;[2]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China

年份：2017

卷号：15

期号：1

起止页码：27-38

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000394568700004)】；

基金：This work was supported by a grant from the China Twelfth Five-Year Plan for Science & Technology Support (2012BAD01B01). The authors thank Prof. Iain Charles Bruce (Peking University, China) for critical reading of the manuscript. The English in this document has also been checked by three professional editors, all native speakers of English. For a certificate, please see https://secure.es.acschemworx.acs.org/certificate/verify, and the certificate verification code is 8EF2-F8AC-B2E9-CE01-9ED7.

语种：英文

外文关键词：Picea asperata; somatic embryo; partial desiccation treatment; proteomics; stress-related protein

摘要：Partial desiccation treatment (PDT) stimulates germination and enhances the conversion of conifer somatic embryos. To better understand the mechanisms underlying the responses of somatic embryos to PDT, we used proteomic and physiological analyses to investigate these responses during PDT in Picea asperata. Comparative proteomic analysis revealed that, during PDT, stress-related proteins were mainly involved in osmosis, endogenous hormones, antioxidative proteins, molecular chaperones and defence-related proteins. Compared with those in cotyledonary embryos before PDT, these stress-related proteins remained at high levels on days 7 (D7) and 14 (D14) of PDT. The proteins that differentially accumulated in the somatic embryos on D7 were mapped to stress and/or stimuli. They may also be involved in the glyoxylate cycle and the chitin metabolic process. The most significant difference in the differentially accumulated proteins occurred in the metabolic pathways of photosynthesis on D14. Furthermore, in accordance with the changes in stress-related proteins, analyses of changes in water content, abscisic acid, indoleacetic acid and H2O2 levels in the embryos indicated that PDT is involved in water-deficit tolerance and affects endogenous hormones. Our results provide insight into the mechanisms responsible for the transition from morphologically mature to physiologically mature somatic embryos during the PDT process in P.asperata.