文献类型：期刊文献

中文题名：盐胁迫下紫花苜蓿萌发期和幼苗期代谢组学分析

英文题名：Metabolomic Analysis of Medicago sativa at Germination and Seedling Stage under Salt Stress

作者：段小兰[1] 朱建峰[2] 李伟[1] 王奎玲[1] 张翠萍[1]

第一作者：段小兰

机构：[1]青岛农业大学园林与林学院,山东青岛266109;[2]中国林业科学研究院生态保护与修复研究所盐碱地研究中心,北京100091

年份：2024

卷号：46

期号：2

起止页码：35-47

中文期刊名：中国草地学报

外文期刊名：Chinese Journal of Grassland

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2019MB008)。

语种：中文

中文关键词：盐胁迫;萌发期;幼苗期;代谢组;紫花苜蓿

外文关键词：Salt stress;Germination stage;Seedling stage;Metabolomics;Medicago sativa

分类号：S541.1

摘要：紫花苜蓿是盐碱地生物改良的优良植物材料,萌发期和幼苗期是其在盐碱地建植的关键时期,但目前对紫花苜蓿盐胁迫下萌发期和幼苗期进行代谢组学分析的研究较少。为全面深入地了解紫花苜蓿的耐盐代谢调控机制,设置0(对照)、50、100、200、400 mmol/L NaCl胁迫,测定紫花苜蓿的出苗率、存活率、株高及根长,并利用高效液相色谱串联质谱(LC-MS/MS)技术分析对照和100 mmol/L NaCl胁迫下紫花苜蓿萌发期和幼苗期根系的代谢变化。结果表明:紫花苜蓿具有一定的耐盐性,在盐浓度≤100 mmol/L时,紫花苜蓿出苗率和存活率与对照相比无显著差异。与对照相比,100 mmol/L盐胁迫下紫花苜蓿萌发期筛选出205个差异代谢物,其中48个差异代谢物富集到55个KEGG代谢通路,差异代谢物主要富集在ABC转运蛋白、磷脂酰肌醇信号系统和氨基酸代谢等通路中,其通路中的差异代谢物肌醇和L-酪氨酸显著上调;紫花苜蓿幼苗期筛选出108个差异代谢物,其中24个差异代谢物富集到37个KEGG代谢通路,差异代谢物主要富集在植物激素信号转导和其他次生代谢产物的生物合成等通路中,其通路中的差异代谢物吲哚-3-乙酸、L-酪氨酸和哌啶酸显著上调。
Alfalfa(Medicago sativa)is an excellent plant material for saline-alkali land bio?improvement.The germination and seedling stages are the key stages for its planting in saline-alkali land.However,there are few reports on the metabonomic analysis of alfalfa under salt stress at the germination and seedling stages.In order to deeply and comprehensively understand the metabolic regulation mecha?nism of salt tolerance in alfalfa,0(control),50,100,200 and 400 mmol/L NaCl stress were set to deter?mine the emergence rate,survival rate,plant height and root length of alfalfa,the root metabolism of alfalfa at germination and seedling stages under control and 100 mmol/L NaCl stress was analyzed by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).The results showed that alfalfa had a certain salt tolerance.When the salt concentration was≤100 mmol/L,there was no signifi?cant difference in seedling emergence rate and survival rate of alfalfa compared to the control treatment.205 differential metabolites were identified at the germination stage of alfalfa under 100 mmol/L salt stress.Among them,48 differential metabolites were enriched in 55 KEGG metabolic pathways.The differential metabolites were mainly enriched in pathways such as ABC transporter,phosphatidylinositol signaling sys?tem,and amino acid metabolism.The differential metabolites inositol and L-tyrosine in these pathways were significantly upregulated.A total of 108 differential metabolites were identified at the seedling stage of alfalfa,of which 24 were enriched in 37 KEGG metabolic pathways.Differential metabolites were mainly enriched in plant hormone signal transduction and biosynthesis of other secondary metabolites.The differential metabolites in these pathways,indole-3-acetic acid,L-tyrosine,and piperidine,were signifi?cantly upregulated.