文献类型：期刊文献

中文题名：植物生长调节剂对黑木相思优树腋芽增殖及生根的影响

英文题名：Effects of Plant Growth Regulators on Axillary Bud Proliferation and Rooting of Acacia melanoxylon Elite Tree

作者：黄烈健[1] 王鸿[1] 胡峰[2] 施琼[1]

第一作者：黄烈健

机构：[1]中国林业科学研究院热带林业研究所;[2]广东省农业科学院作物研究所

年份：2015

卷号：23

期号：6

起止页码：669-674

中文期刊名：热带亚热带植物学报

外文期刊名：Journal of Tropical and Subtropical Botany

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"十二五"科技支撑计划项目(2012BAD01B0402)资助

语种：中文

中文关键词：黑木相思;腋芽增殖;生根;直接器官发生;间接器官发生

外文关键词：Acacia melanoxylon;;Axillary bud proliferation;;Rooting;;Direct organogenesis;;Indirect organogenesis

分类号：S482.8

摘要：为建立黑木相思(Acacia melanoxylon)快繁技术体系,以含1个腋芽的无菌茎段为材料,研究了植物生长调节剂对其增殖和生根的影响。结果表明,6-BA极易诱导黑木相思愈伤组织形成,但芽长势较差,不利于腋芽增殖体系的建立。而生长素既能诱导黑木相思生根,又能诱导腋芽增殖;将无菌茎段接入MS+IAA 0.5 mg L–1+IBA 0.5 mg L–1培养基中培养20 d的生根率为98.41%,培养40 d的腋芽增殖倍数为2.36,单株繁殖系数为6.57。这是首次成功建立高效、简便的生根和增殖同步发生的黑木相思直接器官发生途径的组培技术体系。
In order to establish rapid propagation system of Acacia melanoxylon, the effects of plant growth regulators on its axillary bud multiplication and rooting were studied by using sterile stem segments with one axillary bud as explants. The results showed that the calli of A. melanoxylon were easily induced and bud growth was poor by 6-BA, indicating the disadvantage of axillary bud proliferation. The proliferation and rooting of Acacia melanoxylon axillary buds could be induced by auxin. The stem sections were cultured on MS + IAA 0.5 mg L–1 + IBA 0.5 mg L–1medium, rooting rate was 98.41% cultured for 20 d, and cultured for 40 d, the axillary bud multiplication and propagation coefficient were 2.36 and 6.57 per plantlet, respectively. It was successfully established the direct organogenesis technology system of A. melanoxylon for the first time with the advantages of high efficient, easy rooting and proliferation occur synchronous.