文献类型：期刊文献

英文题名：Isolation and characterisation of a gene for a DREB transcription factor from Phyllostachys edulis induced by drought

作者：Liu, Lei[1,2] Zhao, Hua[2] He, Cong-Fen[2] Bai, Rui[2] Yao, Na[1] Li, Lu-Bin[1]

第一作者：Liu, Lei;刘蕾

通信作者：Li, LB[1]

机构：[1]State Forestry Adm Res, Res Inst Forestry, Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[2]Beijing Technol & Business Univ, Beijing Key Lab Plant Resources Res & Dev, Beijing 100048, Peoples R China

年份：2011

卷号：86

期号：2

起止页码：166-174

外文期刊名：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000289661100013)】；

基金：We thank Professor Shen Shihua (Institute of Plant Botany, Chinese Academy of Science, Beijing, P. R. China) for providing the wild-type and mutant (mDRE) strains of yeast and Professor Ma Youzhi (Chinese Academy of Agricultural Science, Beijing, P. R. China) for providing the vector 163GFP. This work was supported by grants from the Zhejiang Municipal Science and Technology Project (No. 2009C12097) and the Science Foundation of the Research Institute of Subtropical Forestry, Chinese Academy of Forestry (No. RISF6908).

语种：英文

摘要：We have cloned a novel drought-induced gene PeDREB2 from Phyllostachys edulis using the RACE method and demonstrated that the deduced protein sequence contained a conserved AP2 DNA-binding domain that belonged to the EREBP/AP2 family. PeDREB2 gene expression peaked at 12 h under conditions of drought stress, and transcripts accumulated rapidly by 6 h under salt stress, but expression was not affected by abscisic acid or cold stress treatments. In onion epidermal cells, the recombinant protein, PeDREB2-163GFP, was localised to the nucleus. In yeast, the PeDREB2 protein bound specifically to dehydration-response elements to activate transcription of the His3 and LacZ reporter genes. When fused to a sequence encoding the LexA DNA-binding domain, the PeDREB2 protein was an effective trans-activator in the yeast one-hybrid assay. Therefore, PeDREB2 represents a novel transcription factor and, like other EREBP/AP2 regulons, is activated in response to environmental stress factors in tropical plants such as Phyllostachys edulis (bamboo).