文献类型：期刊文献

英文题名：Transcriptomic and proteomic analyses of embryogenic tissues in Picea balfouriana treated with 6-benzylaminopurine

作者：Li, Qingfen[1] Zhang, Shougong[1] Wang, Junhui[1]

第一作者：Li, Qingfen

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Forest Genet & Tree Breeding, Beijing, Peoples R China

年份：2015

卷号：154

期号：1

起止页码：95-113

外文期刊名：PHYSIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353067500008)】；

基金：This research was funded by Advanced Generation Breeding Technological Research and Demonstration of Northern Conifer Species (2012BA D01B01).

语种：英文

摘要：The cytokinin 6-benzylaminopurine (6-BAP) influences the embryogenic capacity of the tissues of Picea balfouriana during long subculture (after 3months). Tissues that proliferate in 3.6 and 5 mu M 6-BAP exhibit the highest and lowest embryogenic capacity, respectively, generating 113 +/- 6 and 23 +/- 3 mature embryos per 100mg of tissue. In this study, a comparative transcriptomic and proteomic approach was applied to characterize the genes and proteins that are differentially expressed among tissues under the influence of different levels of 6-BAP. A total of 51375 unigenes and 2617 proteins were obtained after quality filtering. There were 2770 transcripts for proteins found among these unigenes. Gene ontology (GO) analysis of the differentially expressed unigenes and proteins showed that they were involved in cell and binding activity and were enriched in ribosome and glutathione metabolism pathways. Ribosomal proteins, glutathione S-transferase proteins, germin-like proteins and calmodulin-independent protein kinases were up-regulated in the embryogenic tissues with the highest embryogenic ability (treated with 3.6 mu M 6-BAP), which was validated via quantitative real-time polymerase chain reaction (qRT-PCR) analysis, and these proteins might serve as molecular markers of embryogenic ability. Data are available via Sequence Read Archive (SRA) and ProteomeXchange with identifier SRP042246 and PXD001022, respectively.