文献类型：期刊文献

英文题名：TOWARDS AN EFFICIENT REGENERATION PROTOCOL FOR EUCALYPTUS UROPHYLLA

作者：Li, L. M.[1] Ouyang, L. J.[1] Gan, S. M.[2,3]

第一作者：Li, L. M.

通信作者：Li, LM[1]

机构：[1]Guangdong Univ Petrochem Technol, Maoming 525000, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2015

卷号：27

期号：3

起止页码：289-297

外文期刊名：JOURNAL OF TROPICAL FOREST SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000365202000002)】；

基金：We are grateful to the China Eucalyptus Research Center for providing seeds of E. urophylla. This work was financially supported by the Ministry of Science and Technology of China (2013AA102705). The project was supported by the Open Fund of State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, the National Natural Science Foundation of China (31470677), the Natural Science Foundation of Guangdong Province (S2013040014690) and the Science and Technology Tackle Key Problem (201508) of Maoming.

语种：英文

外文关键词：In-vitro organogenesis; adventitious bud induction; adventitious bud propagation; substituted urea

摘要：The aim of the present study was to establish an efficient regeneration system for Eucalyptus urophylla by means of organogenesis. The hypocotyls from seedlings of E. urophylla clone U6 were used as explants and cultured in a modified Murashige and Skoog (MS) medium, supplemented with 13.2 mu M L-1 N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 mu M L-1 indole-3-acetic acid (IAA). After culture for 5 days, 98.8% explants formed callus. After 30 days, the calli obtained were transferred to Schwarz differential medium (SDM) containing different combinations of 6-benzyladenine (6-BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage (58.6%) of the calli induced by PBU showed adventitious bud formation. Proliferation of adventitious buds was obtained on SDM medium supplemented with 2.2 mu M L-1 6-BA and 0.25 mu M L-1 NAA and shoot elongation was then stimulated on SDM medium supplemented with 5.28 mu M L-1 PBU and 0.25 mu M L-1 NAA for 20 days. For rooting, the elongated shoots were cultivated on root induction medium containing 2.46 mu M L-1 indole-3-butyric acid (IBA). Plantlets were then successfully transplanted to a greenhouse. This paper presents an efficient procedure for regeneration of E. urophylla.