文献类型：期刊文献

中文题名：中国兰属植物菌根真菌的AFLP多样性分析

英文题名：AFLP Diversity in the Mycorrhizal Fungi of Cymbidium Plants

作者：李潞滨[1] 胡陶[1] 杨凯[2] 唐征[2] 庄彩云[1] 刘振静[1] 彭镇华[1]

第一作者：李潞滨

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育实验室,北京100091;[2]北京农学院农业应用新技术北京市重点实验室,北京102206

年份：2008

卷号：35

期号：1

起止页码：81-86

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：‘948’项目(2004-4-26；2005-4-37)

语种：中文

中文关键词：兰属;菌根真菌;AFLP;多样性

外文关键词：Cymbidium ; mycorrhizal fungi ; AFLP; diversity

分类号：S682.31

摘要：对19株分离自不同地理分布和生态型的中国兰属植物菌根共生丝核菌进行了AFLP分析,12对引物组合共扩增出1869条带,其中1016条具有多态性,平均多态性比率达54.4%。AFLP分析结果采用UPGMA聚类,19株菌的相似系数在0.61～0.89之间;聚类关系显示兰花地理分布、所属种和生态类型是影响中国兰属植物菌根真菌多样性及其与兰花间专一性关系的关键性因素。地生型的春兰和云南的附生兰与其共生菌根真菌之间有着较严格的专一性关系。
Amplified fragment length polymorphism （AFLP） was used to study the diversity of 19 representative mycorrhizal Rhizoctonia strains, which isolated from 7 species of Chinese orchids （Cymbidium） distributed in different sites and belonged to different ecologic types. A high degree of diversity was found among the mycorrhizal Rhizoctonia strains. 1 869 bands were got by using 12 pairs of AFLP primers, and among them 1 016 bands were polymorphism, the mean percentage of polymorphism was 54. 4% , which indicated that AFLP was a greatly efficient method to study the diversity of mycorrhizae of Chinese orchids （Cymbidium）. The AFLP results were analyzed for similarity among strains via unweighted pair-group means cluster analysis, similarity among 19 mycorrhizal Rhizoctonia strains ranged corrhizal strains showed 3 crucial factors, the distributed from 0. 61 to 0. 89. Cluster analysis among the myenvironments, orchids species and ecologic type of orchids, which affected the diversity of Chinese orchids （Cymbidium） symbiotic mycorrhizae and the speciality-relation between mycorrhizae and hosts. And the terrestrial orchids belong to Cymbidium goeringii and epibiosis orchids of Yunan Province showed strongly strict speciality-relation with the stains isolated from them.