文献类型：期刊文献

英文题名：ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization

作者：Sang, Jian[1,2,3] Wang, Zhennan[3,4] Li, Man[1,2,3] Cao, Jiabao[1,2,3] Niu, Guangyi[1,2,3] Xia, Lin[1,2,3] Zou, Dong[1,2] Wang, Fan[1,2] Xu, Xingjian[1,2] Han, Xiaojiao[5] Fan, Jinqi[6] Yang, Ye[7] Zuo, Wanzhu[7] Zhang, Yang[1,2,3] Zhao, Wenming[1,3] Bao, Yiming[1,2,3] Xiao, Jingfa[1,2,3,8] Hu, Songnian[2,3,8] Hao, Lili[1,2] Zhang, Zhang[1,2,3,8]

第一作者：Sang, Jian

通信作者：Hao, LL[1];Zhang, Z[1];Hao, LL[2];Zhang, Z[2];Zhang, Z[3];Zhang, Z[4]

机构：[1]Chinese Acad Sci, Beijing Inst Genom, BIG Data Ctr, Beijing 100101, Peoples R China;[2]Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China;[5]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Genom, Hangzhou 311400, Zhejiang, Peoples R China;[6]Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China;[7]Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China;[8]Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Shanghai 200031, Peoples R China

年份：2018

卷号：46

期号：D1

起止页码：D121-D126

外文期刊名：NUCLEIC ACIDS RESEARCH

收录：;Scopus(收录号：2-s2.0-85040932573);WOS:【SCI-EXPANDED(收录号:WOS:000419550700019)】；

基金：Strategic Priority Research Program of the Chinese Academy of Sciences [XDB13040500, XDA08020102 to Z.Z.; XDA08020102 to S.H.]; National Programs for High Technology Research and Development [2015AA020108, 2012AA020409 to Z.Z.]; National Key R&D Program of China [2017YFC0907502 to Z.Z.]; International Partnership Program of the Chinese Academy of Sciences [153F11KYSB20160008]; The 100-Talent Program of Chinese Academy of Sciences [to Y.B. Z.Z.]; National Natural Science Foundation of China [31100915 to L.H.]. Funding for open access charge: Strategic Priority Research Program of the Chinese Academy of Sciences.

语种：英文

摘要：Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and nonmodel organisms.