文献类型：期刊文献

英文题名：Synergism and antagonism: Unraveling the regulatory network of perianth coloration in Dendrobium nobile Lindl.

作者：Wang, Qun[1] Wei, Xin-Rui[1] Yuan, Meng[1] Hou, Tian-Ze[1] Liu, Zhong-Jian[2,3] Zheng, Bao-Qiang[1] Wang, Yan[1]

通信作者：Zheng, BQ[1];Wang, Y[1];Liu, ZJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry & G, Beijing 100091, Peoples R China;[2]Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Natl Forestry & Grassland Adm Orchid Conse, Fuzhou 350002, Peoples R China;[3]Guangzhou Inst Forestry & Landscape Architecture, Guangzhou 510405, Peoples R China

年份：2026

卷号：231

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001673547900001)】；

基金：The research was supported by grants from National Key R&D Program of China (2023YFD1600505) .

语种：英文

外文关键词：Orchid; Dendrobium nobile Lindl.; Perianth coloration; Anthocyanin; MYB transcription factors; MADS-box transcription factors

摘要：Dendrobium nobile Lindl. is a multifunctional orchid species whose perianth diversity is particularly valued in horticulture, yet the molecular mechanisms underlying perianth coloration remain elusive. Here, we compared wild-type (WT), natural mutant with lip-like petals and a purplish-red blotch at the petal base (SC), and natural mutant with lip-like lateral sepals displaying a purplish-red blotch at the sepal base and a petal-like dorsal sepal (DB) of D. nobile to investigate perianth coloration patterns via pigment analysis, transcriptomics, and functional assays. Qualitative and quantitative analysis of pigments revealed that during development, perianth pigmentation shifted from chlorophyll to anthocyanin dominance. DnMYB1, DnMYB2, DnAGL6-2 and DnSEP3 promoted anthocyanin synthesis, as validated by heterologous transient overexpression and virus-induced gene silencing (VIGS). Transcriptomics, yeast one-hybrid (Y1H), and yeast two-hybrid (Y2H) assays revealed a multi-layered regulatory mechanism: DnAGL6-2 and DnSEP3 can directly activate the anthocyanin biosynthesis pathway (ABP) genes DnF3H, DnFLS, and DnBZ1; the spatially specific distribution of DnMYB1 and DnMYB2, the synergistic interaction between DnAGL6-2 and DnMYB1, and the antagonistic interaction between DnAGL6-2 and DnSEP3 collectively determine the coloration difference between the blotch and non-blotch regions. Our study uncovers a regulatory network for perianth coloration driven by the synergistic and antagonistic interactions among transcription factors (TFs), providing new gene targets and strategies for orchid color breeding.