文献类型：期刊文献

英文题名：Chromosome-level genome assembly and population genetic analysis of a near-threatened rosewood species (Dalbergia cultrata Pierre Graham ex Benth) provide insights into its evolutionary and cold stress responses

作者：Huang, Ping[1,2] Li, Changhong[1,2] Lin, Furong[1,2] Liu, Yu[1,2,3] Zong, Yichen[1,2] Li, Bin[1,2] Zheng, Yongqi[1,2]

第一作者：黄平

通信作者：Huang, P[1];Zheng, YQ[1];Huang, P[2];Zheng, YQ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Lab Forest Silviculture & Tree Cultivat, Natl Forestry & Grassland Adm, Beijing, Peoples R China;[3]Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou Key Lab Resource Plant Innovat & Utilizat, Wenzhou, Zhejiang, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001078166100001)】；

基金：This work was supported by the Fund of the National Key Research and Development Program (2022YFD2200100 to ZY), National Natural Science Foundation of China (31761143002 to ZY; 32071783 to PH), Special Funds for the Laboratory of Forest Silviculture and Tree Cultivation, National Forestry and Grassland Administration (No. ZDRIF201713 to ZY).

语种：英文

外文关键词：Dalbergia cultrata; genome assembly; population evolution; cold stress response; rosewood

摘要：Dalbergia cultrata Pierre Graham ex Benth (D. cultrata) is a precious rosewood tree species that grows in the tropical and subtropical regions of Asia. In this study, we used PacBio long-reading sequencing technology and Hi-C assistance to sequence and assemble the reference genome of D. cultrata. We generated 171.47 Gb PacBio long reads and 72.43 Gb Hi-C data and yielded an assembly of 10 pseudochromosomes with a total size of 690.99 Mb and Scaffold N50 of 65.76 Mb. The analysis of specific genes revealed that the triterpenoids represented by lupeol may play an important role in D. cultrata's potential medicinal value. Using the new reference genome, we analyzed the resequencing of 19 Dalbergia accessions and found that D. cultrata and D. cochinchinensis have the latest genetic relationship. Transcriptome sequencing of D. cultrata leaves grown under cold stress revealed that MYB transcription factor and E3 ubiquitin ligase may be playing an important role in the cold response of D. cultrata. Genome resources and identified genetic variation, especially those genes related to the biosynthesis of phytochemicals and cold stress response, will be helpful for the introduction, domestication, utilization, and further breeding of Dalbergia species.