文献类型：期刊文献

英文题名：A novel set of single-copy nuclear gene markers in white oak and implications for species delimitation

作者：Chen, Jun[1,2] Zeng, Yan-Fei[1] Liao, Wan-Jin[3] Yan, Peng-Cheng[4] Zhang, Jian-Guo[1,2]

第一作者：Chen, Jun

通信作者：Zeng, YF[1];Zhang, JG[1];Zhang, JG[2]|[a00054eda5d58f1b4f27e]曾艳飞;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat,State Key Lab Tr, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Jiangsu, Peoples R China;[3]Beijing Normal Univ, MOE Key Lab Biodivers Sci & Ecol Engn, Beijing, Peoples R China;[4]Beijing Comp Ctr, Beijing Key Lab Cloud Comp Key Technol & Applicat, Beijing, Peoples R China

年份：2017

卷号：13

期号：2

外文期刊名：TREE GENETICS & GENOMES

收录：;Scopus(收录号：2-s2.0-85016722255);WOS:【SCI-EXPANDED(收录号:WOS:000400497600020)】；

基金：This work was financially supported by the National Natural Science Foundation of China (31270701, 31370398). We would like to thank Prof. Da-Yong Zhang from Beijing Normal University and Prof. Shou-Xian Li from Taiwan Normal University for their useful discussions and insightful comments in marker development and Dr. Fang Du from Beijing Forestry University for providing the sample of Q. aquifolioides and her help in marker development.

语种：英文

外文关键词：Marker development; Molecular polymorphism; Phylogenetics; Single-copy nuclear gene; Species delimitation; White oak

摘要：Oaks have often been the focus of research on plant evolution owing to their propensity to intercross and their important role in ecology and economy. Compared with traditional molecular markers, such as amplified fragment length polymorphisms (AFLPs) and simple sequence repeats (SSRs), multiple single-copy nuclear genes (SCNGs) are of greater utility in inferring evolutionary processes in oaks. Nineteen primer pairs were developed from expressed sequence tags (ESTs) of Quercus mongolica and Q. robur that could produce orthologous products in Chinese white oaks (section Quercus). These SCNG markers showed a moderate to high level of nucleotide polymorphism in 42 individuals of two closely related white oaks, Q. mongolica and Q. liaotungensis, and demonstrated high transferability across seven white oaks, four oaks from section Cerris, and one oak from section Lobatae. A phylogenetic tree based on these SCNGs provided resolution at deep nodes and robust support for delimiting populations of Q. mongolica and Q. liaotungensis; Bayesian analysis clustered individuals into their respective species with high probability and no admixture. When the same individuals were used, Bayesian clusters based on either 194 AFLPs or 19 SSRs gave comparable results, but one or several individuals respectively were identified as having admixed ancestry. This indicates that the hybridization rate between these two oaks may have been overestimated using SSR markers due to the occurrence of homoplasy. The SCNGs are powerful for species delimitation of white oaks, and these markers could be useful for future phylogenetics and phylogeography research in white oaks.