文献类型：期刊文献

英文题名：Concentration and Distribution of Nuclei and Plastids in Xylem Cells in Cunninghamia lanceolata and Aquilaria sinensis

作者：Zhang, Rong[1,2] Xu, Kuiwu[1] Ye, Kelin[2]

第一作者：Zhang, Rong;张冉

通信作者：Ye, KL[1]

机构：[1]Nanjing Forestry Univ, Coll Mat Sci & Engn, Nanjing, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China

年份：2015

卷号：10

期号：1

起止页码：1304-1317

外文期刊名：BIORESOURCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000351941000105)】；

基金：The authors would like to thank Professor Yin Yafang of the Chinese Research Institute of Wood Industry, the Chinese Academy of Forestry for his valuable comments on experiment and data analysis, Dr. Liu Bo for the guide of confocal microscope, and Mr. Jiao Lichao and Mr. Song Kunlin for collecting the samples. This work was financially supported by a project at the Chinese State Forestry Administration (No. 201304508) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Nuclei; Plastid; Xylem; Aceto-carmine; DAPI; I-2-KI

摘要：After programmed cell death (PCD), heartwood formation, storage, and processing, wood DNA degradation occurs to varying degrees. The concentration and distribution of nuclei and plastids in xylem cells of Cunninghamia lanceolata and Aquilaria sinensis, treated under different conditions of processing and storing, were studied by analyzing the distribution frequency, area, and signal intensity, in specimens that had been stained with aceto-carmine, DAPI, and I-2-KI. Most of the nuclei and plastids were present in the ray cells, and a small quantity of nuclei and plastids were present in the axial parenchyma cells. There was an indication that the concentration of the remaining nuclei and plastids in the xylem cells was mainly affected by the xylem heartwood formation, storage time, and temperature. The nuclei and plastids content of the sapwood was greater than that of the heartwood. However, the nuclei and plastids content of the fresh wood was greater than that of the processed and stored wood. An estimation of the quantity of nuclei and plastids using staining methods could provide a direct basis for the appropriate selection of a procedure for DNA extraction.