文献类型：期刊文献

英文题名：OVER-EXPRESSION OF THE CODA GENE BY RD29A PROMOTER IMPROVES SALT TOLERANCE IN NICOTIANA TABACUM

作者：Jingjiang[1,2] Li, Haiying[1,2] He, Guiping[2] Yin, Yafang[3] Liu, Mingying[1,2] Liu, Bo[2] Qiao, Guirong[1,2] Lin, Sheng[1,2,4] Xie, Lihua[1,2] Zhuo, Renying[1,2]

第一作者：Jingjiang

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Genom, Hangzhou 311400, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[4]Beijing Univ Chem Technol, Beijing 100029, Peoples R China

年份：2013

卷号：45

期号：3

起止页码：821-827

外文期刊名：PAKISTAN JOURNAL OF BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000320305400014)】；

基金：The authors thank Dr. Norio Murata at Department of Regulation Biology, National Institute for Basic Biology (Japan) for kindly providing CodA cDNA. This work was supported by Zhejiang Provincial Natural Science Foundation of China under Grant No. R3090070 and National Non-profit Research Institute of RISF, CAF (No. RISF6155).

语种：英文

摘要：Soil-salinity is a serious problem that impedes food production and reforestation worldwide. In order to improve plant salt tolerance, many genes for abiotic stress have been over-expressed by genetic engineering. The codA gene encodes for the choline oxidase of the soil bacterium Arthrobacter globiformisa. We analyzed the effect of overexpression of CodA on stress tolerance in transgenic tobacco plants using the stress-inducible Rd29A promoter. Four transgenic Nicotiana tabacum plants were obtained. Southern blot analysis demonstrated that the codA gene had integrated into the genome of the tobacco. When exposed to NaCl salt stress, the chlorophyll content of the transgenic plants was not altered significantly. With prolonged stress (2-4 weeks), superoxide dismutase (SOD) activity increased by at least 50% relative to controls in all four transgenic lines. Malondialdehyde (MDA) accumulation was observed after 7 days of NaCl stress in all transgenic plants, but decreased during continued salt stress in some lines, indicative of salt stress tolerance. These results showed that the codA gene may enhance salt-tolerance by inhibiting lipid peroxidation through induction of stress response genes like SOD. These results offer additional support to the suggestion that CodA is involved in the modulation of plant gene activity in response to salt stress conditions and indicate that plants over-expressing codA could be engineered for agriculture in saline soils.