文献类型：期刊文献

中文题名：杉木林土壤和苗木内生溶磷细菌的筛选及其溶磷特性

英文题名：Screening of Phosphate Solubilizing Bacteria from Soil and Endogenous Environment of Chinese Fir Seedlings and Their Characterization of Phosphate Solubilization

作者：韦宜慧[1] 陈嘉琪[1] 赵光宇[1] 董玉红[1] 厚凌宇[1] 焦如珍[1]

第一作者：韦宜慧

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,北京100091

年份：2020

卷号：56

期号：12

起止页码：1-9

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;EI(收录号：20210509871675);Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：中国林业科学研究院中央级公益性科研院所基本科研业务专项资金项目“森林土壤功能微生物种质资源筛选及研究”(CAFYBB2018SY001);国家重点研发计划项目“杉木高效培育技术研究”(2016YFD0600302)。

语种：中文

中文关键词：杉木;溶磷菌;筛选;鉴定;培养条件

外文关键词：Chinese fir;phosphate solubilizing bacteria;screening;identification;culture conditions

分类号：S714.3

摘要：【目的】筛选和鉴定杉木高效溶磷菌,研究其溶磷特性,探讨其生态适应性和溶磷特点,为研制杉木适用的微生物肥料提供菌种资源和培养条件。【方法】利用PVK无机磷培养基从杉木林地土壤和杉木植株分离溶磷菌,筛选出高效溶磷菌并结合生理生化试验和16S rDNA基因序列对其进行鉴定,采用单因素试验分析高效溶磷菌在不同pH、温度、碳源、氮源、磷源等培养条件下的溶磷特性。【结果】1)共分离筛选出20株溶磷菌,其中溶磷能力最强的3株菌株为P5、RP2和RP22(分别为195.61 mg·L^-1、109.20 mg·L^-1、78.86 mg·L^-1)。2)通过生理生化试验,结合16S rDNA序列分析及系统发育树的构建,菌株P5鉴定为Burkholderia ubonensis,RP2为Pseudomonas frederiksbergensis,RP22为Pseudomonas grimontii。3)单因素试验溶磷培养条件研究结果显示,P5菌株在pH为5,温度为30℃,碳源为蔗糖,氮源为氯化铵,磷源为磷酸钙时溶磷能力最强;RP2菌株在pH为7,温度为30℃,碳源为葡萄糖,氮源为硝酸钾,磷源为磷酸钙时溶磷能力最强;RP22菌株在pH为7,温度为25℃,碳源为葡萄糖,氮源为硝酸钾,磷源为磷酸钙时溶磷能力最强。【结论】所筛选溶磷菌具有高效溶磷能力,其中菌株P5在酸性和高温条件下仍有较高的溶磷能力,在微生物肥料研制中具有巨大潜力。
【Objective】This study aimed to screen and identify efficient phosphate solubilizing bacteria from Chinese fir,We investigated its phosphate solubilizing characteristics,and explored ecological adaptability,so as to provide bacterial resources and culture conditions for the development of microbial fertilizer for Chinese fir.【Method】Phosphate solubilizing bacteria were isolated from soil and Chinese fir plant by PVK inorganic phosphorus medium,and identified by physiological and biochemical tests as well as 16S rDNA gene sequence.A single factor test was used to analyze the phosphate solubilizing characteristics of phosphate solubilizing bacteria under different pH,temperature,carbon source,nitrogen source and phosphorus source.【Result】1)A total of 20 strains of phosphate solubilizing bacteria were isolated and screened,and the three strains with the strongest phosphate solubilizing capacity were P5,RP2 and RP22(195.61,109.20 and 78.86 mg·L^-1,respectively).2)Strain P5 was identified as Burkholderia ubonensis,RP2 as Pseudomonas frederiksbergensis and RP22 as Pseudomonas grimontii by physiological and biochemical tests,combined with 16S rDNA sequence analysis and phylogenetic tree construction.3)The results of single factor experiment showed that the P5 strain had the strongest phosphate solubilizing ability when the pH was 5,the temperature was 30℃,the carbon source was sucrose,the nitrogen source was ammonium chloride,and the phosphorus source was calcium phosphate.RP2 showed the strongest phosphate solubilizing ability when pH7,30℃,the carbon source was glucose,the nitrogen source was potassium nitrate,and the phosphorus source was calcium phosphate.RP22 showed the strongest phosphate solubilizing ability when the pH7,25℃,the carbon source was glucose,the nitrogen source was potassium nitrate,and the phosphorus source was calcium phosphate.【Conclusion】The screened bacteria have high phosphate solubilizing ability among which strain P5 has an efficient phosphate solubilizing ability under acidic and high temperature conditions,which has great potential for the development of microbial fertilizers.