文献类型：期刊文献

英文题名：Identification and characterization of two O-methyltransferases involved in biosynthesis of methylated 2-(2-phenethyl)chromones in agarwood

作者：Wu, Wenli[1] Yan, Tingting[2] Sun, Xiaochen[1] Wilson, Iain[3] Li, Gaiyun[2] Hong, Zhou[4] Shao, Fenjuan[1] Qiu, Deyou[1]

第一作者：Wu, Wenli

通信作者：Shao, FJ[1];Qiu, DY[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry & G, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[3]CSIRO Agr & Food, Canberra, ACT, Australia;[4]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

年份：2024

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85195647299);WOS:【SCI-EXPANDED(收录号:WOS:001209765300001)】；

基金：This work was supported by the Special Funds for Central Public-Interest Scientific Institution Public Welfare Projects (CAFYBB2021QD003).

语种：英文

外文关键词：Aquilaria sinensis; drilling treatment; expression pattern; functional characterization; methylated 2-(2-phenethyl)chromones; O-methyltransferases

摘要：The 2-(2-phenethyl)chromones (PECs) are the signature constituents responsible for the fragrance and pharmacological properties of agarwood. O-Methyltransferases (OMTs) are necessary for the biosynthesis of methylated PECs, but there is little known about OMTs in Aquilaria sinensis. In this study, we identified 29 OMT genes from the A. sinensis genome. Expression analysis showed they were differentially expressed in different tissues and responded to drill wounding. Comprehensive analysis of the gene expression and methylated PEC content revealed that AsOMT2, AsOMT8, AsOMT11, AsOMT16, and AsOMT28 could potentially be involved in methylated PECs biosynthesis. In vitro enzyme assays and functional analysis in Nicotiana benthamiana demonstrated that AsOMT11 and AsOMT16 could methylate 6-hydroxy-2-(2-phenylethyl)chromone to form 6-methoxy-2-(2-phenylethyl)chromone. A transient overexpression experiment in the variety 'Qi-Nan' revealed that AsOMT11 and AsOMT16 could significantly promote the accumulation of three major methylated PECs. Our results provide candidate genes for the mass production of methylated PECs using synthetic biology. Two O -methyltransferases were characterized that are involved in biosynthesis of methylated 2-(2-phenethyl)chromones responsible for the fragrance and pharmacological properties of agarwood.