详细信息
Comparative genomics analysis reveals gene family expansion and changes of expression patterns associated with natural adaptations of flowering time and secondary metabolism in yellow Camellia ( SCI-EXPANDED收录) 被引量:13
文献类型:期刊文献
英文题名:Comparative genomics analysis reveals gene family expansion and changes of expression patterns associated with natural adaptations of flowering time and secondary metabolism in yellow Camellia
作者:Li, Xinlei[1,2] Fan, Zhengqi[1,2] Guo, Haobo[3,4] Ye, Ning[5] Lyu, Tao[1,6] Yang, Wen[1,2] Wang, Jie[1,2] Wang, Jia-Tong[1] Wu, Bin[1,2] Li, Jiyuan[1,2] Yin, Hengfu[1,2]
第一作者:李辛雷
通信作者:Yin, HF[1];Yin, HF[2]
机构:[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Fuyang 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Key Lab Forest Genet & Breeding, Fuyang 311400, Zhejiang, Peoples R China;[3]Univ Tennessee, Coll Engn Sci, SimCtr, Chattanooga, TN 37403 USA;[4]Univ Tennessee, Coll Comp Sci, SimCtr, Chattanooga, TN 37403 USA;[5]Nanjing Forestry Univ, Southern Modern Forestry Collaborat Innovat Ctr, Nanjing 210037, Jiangsu, Peoples R China;[6]Ningbo Univ, Coll Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
年份:2018
卷号:18
期号:6
起止页码:659-671
外文期刊名:FUNCTIONAL & INTEGRATIVE GENOMICS
收录:;WOS:【SCI-EXPANDED(收录号:WOS:000447225700004)】;
基金:This work was supported by Nonprofit Research Projects (CAFYBB2017ZX001-7, CAFYBB2016SZ001) of Chinese Academy of Forestry, and National Science Foundation of China (NSFC) Grant 31470697. We also acknowledge the supports from the Zhejiang province science and technology plan (2013C32075) and International Sci. & Tech. Cooperation Program of China (2016YFE0126100). We thank Prof. Tongming Yin from Nanjing Forestry University (Nanjing, China) for the help of YellowCamelliaDB web portal.
语种:英文
外文关键词:Comparative genomics; Gene expression; Gene family; Camellia; Trait adaptation; Floral pigmentation
摘要:Yellow-flowering species are unique in the genus Camellia not only for their bright yellow pigments but also the health-improving substances in petals. However, little is known regarding the biosynthesis pathways of pigments and secondary metabolites. Here, we performed comparative genomics studies in two yellow-flowered species of the genus Camellia with distinctive flowering periods. We obtained 112,190 and 89,609 unigenes from Camellia nitidissima and Camellia chuongtsoensis, respectively, and identified 9547 gene family clusters shared with various plant species and 3414 single-copy gene families. Global gene expression analysis revealed six comparisons of differentially expressed gene sets in different developmental stages of floral bud. Through the identification of orthologous pairs, conserved and specific differentially expressed genes (DEGs) between species were compared. Functional enrichment analysis suggested that the gibberellin (GA) biosynthesis pathway might be related to the alteration of flowering responses. Furthermore, the expression patterns of secondary metabolism pathway genes were analyzed between yellow- and red-flowered Camellias. We showed that the key enzymes involved in glycosylation of flavonoids displayed differential expression patterns, indicating that the direct glycosylation of flavonols rather than anthocyanins was pivotal to coloration and health-improving metabolites in the yellow Camellia petals. Finally, the gene family analysis of UDP-glycosyltransferases revealed an expansion of group C members in C. nitidissima. Through comparative genomics analysis, we demonstrate that changes of gene expression and gene family members are critical to the variation of natural traits. This work provides valuable insights into the molecular regulation of trait adaptations of floral pigmentation and flowering timing.
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