文献类型：期刊文献

英文题名：Adaptive Pathways of Microorganisms to Cope With the Shift From P- to N-Limitation in Subtropical Plantations

作者：Wang, Chaoqun[1,2,3] Jiao, Ruzhen[1,2,3]

第一作者：Wang, Chaoqun

通信作者：Jiao, RZ[1];Jiao, RZ[2];Jiao, RZ[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[3]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85130280891);WOS:【SCI-EXPANDED(收录号:WOS:000795645000001)】；

基金：Funding This study was supported by the State Key Research Development Program of China (2016YFD0600302).

语种：英文

外文关键词：C; N; P ratio; stoichiometric imbalances; ecological stoichiometry; microbial community; enzymatic activity; microbial nutrient limitation

摘要：Ecological stoichiometry is increasingly acknowledged as one of the main control factors for microbial activity and diversity. Soil carbon/nitrogen (C/N) and carbon/phosphorus (C/P) ratios are usually much higher than microbial nutrient requirements and vary with planting density and stand age in forestlands. However, how microorganisms cope with such stoichiometric imbalances and how they regulate nutrient cycling remain unclear. Here, 5- and 35-year-old experimental Cunninghamia lanceolata [Lamb.] Hook plantations with five planting densities (1,667, 3,333, 5,000, 6,667, and 10,000 stems ha(-1)) were used to explore the underlying mechanism of the response of microorganisms to stoichiometric imbalances. We found that (i) enzyme activity and microbial biomass and diversity increased with planting density at age 5 but decreased at age 35; (ii) soil microorganisms were P-limited at age 5, but gradually shifted from P- to N-limitation during the development of plantations from 5 to 35 years; and (iii) significantly negative relationships between microbial biomass stoichiometry and enzymatic stoichiometry were observed. The adaptive pathways of soil microorganisms to cope with stoichiometric imbalances include (i) adjusting the stoichiometry of microorganisms and enzymes; (ii) changing the relative abundance of the dominant microbial phyla; and (iii) increasing the ratio of fungal to bacterial diversity. These results highlight how to use the ecological stoichiometry method to identify soil microbial nutrient limitations with planting density during the development of plantations. By underlining the important role of stoichiometry on microbial growth and activity, these findings furthermore emphasize the dependency of organic matter transformation and nutrient cycling on the microbial community.