文献类型：期刊文献

英文题名：Effects of Donor Ages and Propagation Methods on Seedling Growth of Platycladus orientalis (L.) Franco in Winter

作者：Dong, Yao[1] Xiao, Wenfa[1] Guo, Wei[2] Liu, Yifu[1] Nie, Wen[1] Huang, Ruizhi[1] Tan, Cancan[1] Jia, Zirui[3] Liu, Jianfeng[3] Jiang, Zeping[1] Chang, Ermei[3]

第一作者：Dong, Yao

通信作者：Chang, EM[1]

机构：[1]Chinese Acad Forestry, Ecol & Nat Conservat Inst, Key Lab Forest Ecol & Environm, Natl Forestry & Grass Land Adm, Beijing 100091, Peoples R China;[2]Taishan Acad Forestry Sci, Tai An 271000, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry & G, Beijing 100091, Peoples R China

年份：2023

卷号：24

期号：8

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000977723300001)】；

基金：This study was financed by the National Key Research and Development Program of China (2022YFD2200103), National Natural Science Foundation of China (31870664), and open fund of the State Key Laboratory of Subtropical Silviculture (SKLSS-KF2022-09).

语种：英文

外文关键词：arborvitae; ancient trees; propagation methods; low temperature; transcriptome

摘要：To evaluate the effects of donor ages on growth and stress resistance of 6-year-old seedlings propagated from 5-, 2000-, and 3000-year-old Platycladus orientalis donors with grafting, cutting, and seed sowing, growth indicators and physiological and transcriptomic analyses were performed in 6-year-old seedlings in winter. Results showed that basal stem diameters and plant heights of seedlings of the three propagation methods decreased with the age of the donors, and the sown seedlings were the thickest and tallest. The contents of soluble sugar, chlorophyll, and free fatty acid in apical leaves of the three propagation methods were negatively correlated with donor ages in winter, while the opposite was true for flavonoid and total phenolic. The contents of flavonoid, total phenolic, and free fatty acid in cutting seedlings were highest in the seedlings propagated in the three methods in winter. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of differentially expressed genes showed phenylpropanoid biosynthesis and fatty acid metabolism pathways, and their expression levels were up-regulated in apical leaves from 6-year-old seedlings propagated from 3000-year-old P. orientalis donors. In addition, hub genes analysis presented that C4H, OMT1, CCR2, PAL, PRX52, ACP1, AtPDAT2, and FAD3 were up-regulated in cutting seedlings, and the gene expression levels decreased in seedlings propagated from 2000- and 3000-year-old donors. These findings demonstrate the resistance stability of cuttings of P. orientalis and provide insights into the regulatory mechanisms of seedlings of P. orientalis propagated from donors at different ages in different propagation methods against low-temperature stress.