文献类型：期刊文献

英文题名：Assembly and phylogenetic analysis of the complete mitochondrial genome of a widely planted hybrid eucalypt (Eucalyptus urophylla x E. grandis)

作者：Li, Chenhe[1] Wang, Jing[1] Fan, Chunjie[2] Kang, Xiangyang[1] Yang, Jun[1]

第一作者：Li, Chenhe

通信作者：Yang, J[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat,Mi, State Key Lab Tree Genet & Breeding,Key Lab Genet, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Key Lab State Forestry & Grassland Adm Trop Forest, Guangzhou 510520, Peoples R China

年份：2025

卷号：36

期号：1

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001488136600001)】；

基金：This research was supported by the Fundamental Research Funds for the Central Universities (2021ZY62), the National Natural Science Foundation of China (31901337) and the 55 Engineering Research & Innovation Team Project of Beijing Forestry University (BLRC2023C06).

语种：英文

外文关键词：Eucalyptus urophylla x E. grandis; Myrtaceae; Mitochondrial genome; Repetitive sequences; RNA editing; Phylogenetic relationship

摘要：Eucalyptus urophylla x E. grandis is a major hybrid species of timber plantations. However, our understanding of Eucalyptus mitochondrial genome, especially within the Myrtaceae family, is limited. In this study, we employed hybrid sequencing combining the Illumina and Oxford Nanopore sequencing to assemble and annotate the mitogenome (mtDNA) of E. urophylla x E. grandis. Our results reveal a structure characterized by one circular molecule, with a cumulative length of 483,907 base pairs (bp) and a GC content of 44.96%. The circular molecule collectively harbored 59 annotated genes. Among these, 38 were unique protein-coding genes (PCGs), accompanied by 18 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. Our study also examined repetitive sequences, RNA editing sites, and intracellular sequence transfers within the mtDNA. Furthermore, we conducted a phylogenetic analysis between E. urophylla x E. grandis and 30 closely related species based on genetic affinities. The outcomes furnish a high-quality organelle genome for E. urophylla x E. grandis, thereby explaining basic insights into organelle genome evolution and phylogenetic relationships.