文献类型：期刊文献

中文题名：黑杨应答杨生褐盘二孢专化型侵染的基因差异表达

英文题名：Gene Differential Expression on Populus deltoids Responding to Infection by Marssonina brunnea Formae

作者：王凯英[1] 高茜[1] 孙晓明[1] 张琰锋[2] 严东辉[1,3]

第一作者：王凯英

机构：[1]中国林业科学研究院森林生态环境与保护研究所;[2]北京林业大学林学院;[3]南京林业大学南方现代林业协同创新中心

年份：2017

卷号：30

期号：4

起止页码：582-587

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：基金项目:效应物在杨盘二孢半活养方式及亲和寄主致病性中的作用(31370645);水解纤维素产烃树木内生菌资源利用技术引进(2013-4-10);芯片通量病原检测技术在杨树病害防控中的应用([2016]10号)

语种：中文

中文关键词：杨生褐盘二孢;专化型;RT-qPCR;基因表达

外文关键词：Marssonina brunnea; formae speciales; Real-time PCR, gene express

分类号：S796

摘要：[目的]杨生褐盘二孢的单芽管专化型和多芽管专化型分别引起白杨组和黑杨组(包括青杨组)杨树的褐斑病,其分化机制有待阐明。[方法]本文在专化性互作体系建立的基础上,利用荧光染色标记研究了病菌孢子在专化性寄主侵染过程中的萌发发育情况,并通过RT-qPCR分析了黑杨寄主抗病相关基因在应答两专化型侵染过程中的不同表达。[结果]结果发现多芽管分生孢子在黑杨寄主上能完成发育并成功侵染,单芽管分生孢子也能成功萌发和发育,但不能侵入黑杨寄主;黑杨抗性基因在两专化型侵染发育过程中均能被诱导表达,但在表达时间和表达量上,除基因WRKY89的表达较为一致外,病程相关基因PR5、PR10、NPR1和LAR3在二者间的表达存在差异。[结论]研究结果初步表明病原专化型可能是寄主与病原专性互作的表型,这一认识将有助于进一步探明杨树褐斑病病原专化型形成和分化的机制。
[Objective] To study the mechanism of Marssonina brunnea sp. monogermtubi formae speciales （RHBH） infecting Populus Section Leuce and M. brunnea sp. multigermtubi formae speciales （RHHB） infecting Populus Section Aigeiros. [Method] Based on pathogen-host interaction system, the pathogen＇s spore germination, growth and infection process on poplar leaves were investigated by using fluorescence microscopy strain method and the expression of five pathogen-relative genes in hosts was also analyzed by RT-qPCR technique. [Result] The results showed that the spores of RHHB were able to grow and successfully infect the hosts of Populus Section Aigeiros, the spores of RHBH were also able to grow but failed to infect the hosts of Populus Section Aigeiros. The pathogen-resistant genes of Populus Section Aigeiros could be induced and express during the infection process of the two formae speciales. The gene express of WRIKY89 followed a similar expression trend, while the other four genes （PR5, PR10, NPR1 and LAR3） followed different expression patterns in time and quantity for the two formae speciales. [Conclusion] The study provides some references for study the mechanisms of the two formae speciales and their differentiation.