文献类型：期刊文献

中文题名：木麻黄青枯病抗性鉴定方法比较及抗病种质筛选

英文题名：Comparison of identification methods of bacterial wilt resistance in Casuarinaceae and screening of resistant germplasm resources

作者：许秀玉[1,2] 张卫强[2] 黄钰辉[2] 甘先华[2] 仲崇禄[3] 张华新[1]

第一作者：许秀玉

机构：[1]中国林业科学研究院国家林业局盐碱地研究中心;[2]广东省森林培育与保护利用重点实验室/广东省林业科学研究院;[3]中国林业科学研究院热带林业研究所

年份：2017

卷号：38

期号：4

起止页码：87-94

中文期刊名：华南农业大学学报

外文期刊名：Journal of South China Agricultural University

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：广东省林业科技创新项目(2014KJCX017;2013KJCX018-03)

语种：中文

中文关键词：木麻黄;青枯病;抗病性;鉴定方法;种质资源

外文关键词：Casuarinaceae ; bacterial wilt ; disease resistance ; identification method ; germplasm resource

分类号：S432.4

摘要：【目的】筛选出简便、准确、可靠的木麻黄(Casuarinaceae)青枯病抗性鉴定方法,对我国现有的木麻黄种质资源开展抗病性鉴定与评价,筛选出优良抗病无性系。【方法】在木麻黄抗病品系筛选技术基础上,参考番茄Lycopersicon esculentum、烟草Nicotiana及桉树Eucalyptus等植物青枯病抗性鉴定方法,设计8种木麻黄青枯菌人工接种方法,系统探讨水培生根苗、嫩枝、绿梗小枝、褐梗小枝、青枯菌粗毒素及盆栽小苗伤根接种、盆栽小苗无伤接种等接种方法对木麻黄青枯病抗性鉴定的影响。【结果】不同无性系利用盆栽幼苗伤根接种法接种后的死亡率介于25.79%~83.06%,无性系K18、A14与G1、30差异极显著(P<0.05);不同无性系用褐梗小枝室内水培接种的病情指数介于2.16~69.48,无性系K18、A14与G1、30差异极显著(P<0.05)。这2种接种方法能够有效区分木麻黄抗、感无性系,且呈极显著正相关(r=0.856 5)。不同无性系在盆栽幼苗不伤根接种及水培生根小苗、嫩枝室内水培接种中抗病性差异不显著(P>0.05),不能有效区分抗、感无性系。绿梗小枝接种后症状变化小,不易分级,容易出现观测误差;青枯菌粗毒素接种存在浓度难以控制等问题。利用褐梗小枝室内水培接种法,对53份木麻黄育种材料进行抗性评价,筛选出X1、30、杂交、G1等12份高抗材料。【结论】盆栽幼苗伤根接种及褐梗小枝室内水培接种方法是木麻黄青枯病抗性鉴定的较好方法,文中其他接种方法不是木麻黄青枯病抗性鉴定的优选方法。
【Objective】 To select convenient, accurate and reliable identification methods of bacterial wilt resistance in Casuarinaceae, identify and evaluate the bacterial wilt resistance of Casuarinaceae germplasm resources in China, and screen out highly resistant clones.【Method】Based on the screening techniques of Casuarinaceae resistant lines as well as the identification methods of bacterial wilt in other plants, such as Lycopersicon esculentum, Nicotiana and Eucalyptus, we designed eight different approaches to inoculate Ralstonia solanacearum into Casuarinaceae. The effects of using hydroponic rooting seedling, twig, lignified green branch, lignified brown branch, bacterial wilt crude toxin, and root with or without injure of potted seedlings in inoculation on bacterial wilt resistance identification were studied. 【Result】After injured root inoculation of potted seedling,the mortalities of different Casuarinaceae clones ranged from 25.79% to 83.06% with significant differences among A14, K18 and G1, 30 clones（P〈0.05）. After hydroponic inoculation of lignified brown branch,the disease indexes of different clones ranged from 2.16 to 69.48 with significant differences among A14, K18 and G1, 30 clones（P〈0.05）. These two inoculation approaches enabled effective discrimination between resistant and infected clones, and had highly significant positive correlation （r=0.856 5）. Non-injured root inoculation of potted seedling, hydroponic inoculation of rooting seedling and tender branch did not result in significantly differences in disease resistance among clones（P〉0.05）, and they couldn’t help with effective identification of bacterial wilt resistance in Casuarinaceae. Inoculation of lignified green branch only resulted in small differences among clones, and therefore it led to difficulties in classification and could easily cause detection error. The concentration of bacterial wilt crude toxin was hard to control during inoculation. Using hydroponic inoculation of lignified brown branch, 53 Casuarinaceae clones were evaluated and 12 highly resistant clones such as X1, 30, hybrid and G1 clones were screened out. 【Conclusion】 Injured root inoculation of potted seedling and hydroponic inoculation of lignified brown branch are both preferred inoculation approaches for identifying bacterial wilt resistance of Casuarinaceae. The rest inoculation approaches tested in this study were not suitable for identification of bacterial wilt resistance in Casuarinaceae.