文献类型：期刊文献

英文题名：Assessment of Epigenetic and Phenotypic Variation in Populus nigra Regenerated via Sequential Regeneration

作者：Zhang, Weixi[1,2] Wang, Yanbo[1,2,3] Diao, Shu[1,2] Zhong, Shanchen[1,2] Wu, Shu[1,2] Wang, Li[1,2] Su, Xiaohua[1,2] Zhang, Bingyu[1,2]

第一作者：张伟溪

通信作者：Zhang, BY[1];Zhang, BY[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, Beijing, Peoples R China;[3]Nanchang Inst Technol, Nanchang, Jiangxi, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000674734900001)】；

基金：The financial support of this study was from the Basic Research Fund of RIF (Grant No. CAFYBB2020SZ002) and the National Natural Science Foundation of China (Grant Nos. 31700589 and 31770710).

语种：英文

外文关键词：sequential regenerants; MSAP; DNA methylation; epigenetic variation; Populus nigra

摘要：Somatic variation has been demonstrated in tissue culture regenerated plants of many species. In the genus Populus, phenotypic variation caused by changes in 5-methylcytosine within the plant genome have been reported. To date, the phenotypic and epigenetic stability of plants regenerated from sequential regeneration has not been tested in trees. In this study, we detected DNA methylation of CCGG sites in regenerated plants of five generations in Populus nigra using methylation-sensitive amplified polymorphisms, and evaluated their growth performance and physiological traits. About 10.86-26.80% of CCGG sites in the regenerated plant genome were demethylated and 5.50-8.45% were methylated, resulting in significantly lower DNA methylation levels among all regenerated plants than among donor plants. We detected a significant difference in methylation levels between first regeneration regenerated plants (G1) and those of the other four generations (G2-G5); there were no significant differences among the four later generations. Therefore, the dramatic decrease in DNA methylation levels occurred only in the first and second poplar regenerations; levels then stabilized later in the regeneration process, indicating that two regeneration events were sufficient to change the methylation statuses of almost all CCGG sites sensitive to regeneration. Differences in growth and physiological traits were observed between regenerated plants and donor plants, but were significant only among plants of certain generations. Significant correlations were detected between methylation level and transpiration rate, net photosynthetic rate, peroxidase activity, and instant water utilization efficiency, indicating the involvement of epigenetic regulation in this unpredictable phenotypic variation.