文献类型：期刊文献

英文题名：Assembly and comparative analysis of the complete mitochondrial genome of Salix wilsonii using PacBio HiFi sequencing

作者：Han, Fuchuan[1,2] Qu, Yanshu[1] Chen, Yicun[2] Xu, Li'an[1] Bi, Changwei[1,3]

第一作者：Han, Fuchuan

通信作者：Bi, CW[1];Bi, CW[2]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forestry Genet & Biotechnol, Minist Educ, Nanjing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[3]Nanjing Forestry Univ, Coll Informat Sci & Technol, Nanjing, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000891562100001)】；

基金：Funding The work is supported by the Natural Science Foundation of Jiangsu Province~(BK20220414) and the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province. The work is also supported by the National Key Research and Development Program of China (2021YFD2201205) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Salix wilsonii; mitochondrial genome; comparative analysis; HiFi sequencing; assembly

摘要：Salix L. (willows) is one of the most taxonomically complex genera of flowering plants, including shrubs, tall trees, bushes, and prostrate plants. Despite the high species diversity, only five mitochondrial genomes (mitogenomes) have been released in this genus. Salix wilsonii is an important ornamental and economic willow tree in section Wilsonia of the genus Salix. In this study, the S. wilsonii mitogenome was assembled into a typical circular structure with a size of 711,456 bp using PacBio HiFi sequencing. A total of 58 genes were annotated in the S. wilsonii mitogenome, including 33 protein-coding genes (PCGs), 22 tRNAs, and 3 rRNAs. In the S. wilsonii mitogenome, four genes (mttB, nad3, nad4, and sdh4) were found to play important roles in its evolution through selection pressure analysis. Collinearity analysis of six Salix mitogenomes revealed high structural variability. To determine the evolutionary position of S. wilsonii, we conducted a phylogenetic analysis of the mitogenomes of S. wilsonii and 12 other species in the order Malpighiales. Results strongly supported the segregation of S. wilsonii and other five Salix species with 100% bootstrap support. The comparative analysis of the S. wilsonii mitogenome not only sheds light on the functional and structural features of S. wilsonii but also provides essential information for genetic studies of the genus Salix.