文献类型：期刊文献

英文题名：Identification and Characterization of Novel MicroRNAs from Populus cathayana Rehd

作者：Zhou, Jing[1] Zhuo, Renying[1] Liu, Mingying[1] Qiao, Guirong[1] Jiang, Jing[1] Li, Haiying[1] Qiu, Wenmin[1] Zhang, Xiaoguo[1] Lin, Shen[1]

通信作者：Zhuo, RY[1]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, Key Lab Tree Genom, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2011

卷号：29

期号：1

起止页码：242-251

外文期刊名：PLANT MOLECULAR BIOLOGY REPORTER

收录：;Scopus(收录号：2-s2.0-79251611109);WOS:【SCI-EXPANDED(收录号:WOS:000286674600025)】；

基金：We would like to thank Dr. Han-Jiang Fu of the Academy of Military Medical Science for technical assistance and Dr. Zhangxun Wang in the Tongji University School of Medicine. This work was supported by the National Natural Science Fundation of China (30972340) and Zhejiang Provincial Natural Science Foundation of China under Grant No. R3090070.

语种：英文

外文关键词：Novel miRNA; miRNA Library; P. cathayana Rehd; Expression; Target genes

摘要：MicroRNAs (miRNAs) are highly conserved, endogenous, non-coding small RNAs of about 18-24 nucleotides in length. As negative regulators, miRNAs inhibit gene expressions at the post-transcriptional level by guiding cleavage or attenuating the translation of target genes, which affects organismal morphogenesis, development, and adaptability to changes occurring in the environment. Although miRNAs have been found in Populus species and have been shown to have diverse biological functions, few have been described in Populus cathayana Rehd. In this study, we have identified novel miRNAs from P. cathayana by constructing a library of small (18-24 nucleotides) RNA. After sequencing 691 small RNAs from P. cathayana, alignment to the Populus sequence database and secondary structure prediction were performed. These bioinformatic analyses identified five candidate miRNAs that were designated ptc-miR801, ptc-miR74, ptc-miR102, ptc-miR367, and ptc-miR213. Expression of these miRNAs was validated using real-time quantitative PCR analysis, and four of them displayed distinct expression patterns in leaves, stems, and roots. Our results show that four of the miRNAs are likely specific to leaves. Putative targets for these miRNAs play important roles in development and response to abiotic stress.