文献类型：期刊文献

英文题名：Reference gene selection for quantitative real-time polymerase chain reaction in Populus

作者：Xu, Meng[1,2] Zhang, Bo[1,2] Su, Xiaohua[3] Zhang, Shougong[3] Huang, Minren[1,2]

第一作者：Xu, Meng

通信作者：Huang, MR[1]

机构：[1]Nanjing Forestry Univ, Jiangsu Key Lab Poplar Germplasm Enhancement & Va, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Key Lab Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2011

卷号：408

期号：2

起止页码：337-339

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000284562700022)】；

基金：This work was supported by the National Basic Research Program of China (2009CB19100).

语种：英文

摘要：Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.